Quantification of hyaluronan in pharmaceutical formulations using high performance capillary electrophoresis and the modified uronic acid carbazole reaction

被引：30
|
作者：
Plätzer, M
Ozegowski, JH
Neubert, RHH
机构：
[1] Univ Halle Wittenberg, Dept Pharm, Inst Pharmaceut & Biopharmaceut, D-06120 Halle, Germany
[2] Univ Jena, Inst Expt Microbiol, D-07745 Jena, Germany
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1999年 / 21卷 / 03期
关键词：
hyaluronan; capillary electrophoresis; carbazole reaction;
D O I：
10.1016/S0731-7085(99)00120-X
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The amount of hyaluronan (HA) in pharmaceutical formulations was determined by high-performance capillary electrophoresis (HPCE) and the results were compared with the carbazole reaction established by Bitter and Muir (T. Bitter, H.M. Muir, Anal. Biochem. 4 (1962) 330-334), HA analysis was performed in less than 10 min by using an untreated fused silica capillary with bubble detection cell. The influence of several buffers and pH values was examined. Calibration curve shows good linearity from 0.01 to 5.0 mg/ml. The lower limit of detection by monitoring the absorbance at 195 nm was 10 mu g/ml at a signal to noise ratio of 5. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：491 / 496
页数：6
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