Detection of MUM1/IRF4-IgH fusion in multiple myeloma

被引：57

作者：

Yoshida, S

Nakazawa, N

Iida, S

Hayami, Y

Sato, S

Wakita, A

Shimizu, S

Taniwaki, M

Ueda, R

机构：

[1] Nagoya City Univ, Sch Med, Dept Internal Med 2, Mizuho Ku, Nagoya, Aichi 4678601, Japan

[2] Kyoto Prefectural Univ Med, Dept Internal Med 3, Kyoto 602, Japan

[3] Shimane Prefectural Cent Hosp, Izumo, Shimane, Japan

来源：

LEUKEMIA | 1999年 / 13卷 / 11期

关键词：

MUM1/IRF4; multiple myeloma; t(6; 14)(p25; q32); IgH insertion; FISH; PFGE;

D O I：

10.1038/sj.leu.2401563

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

MUM1 (multiple myeloma oncogene 1)/IRF4 (interferon regulatory factor 4) gene has been identified as an oncogene transcriptionally activated by t(6;14)(p25;q32) chromosomal translocation in multiple myeloma (MFA). The significance of this alteration in MM remains unknown, as it is not detectable by means of conventional cytogenetic analysis. To address this issue, we established diagnostic procedures based on pulsed-field gel electrophoresis (PFGE) analysis and double color fluorescence in site hybridization (DCFISH) using DNA probes derived from the MUM1 and the immunoglobulin heavy chain (IgH) gene loci. Among a panel of 17 MM cell lines, three (17.6%) showed fusions between these two loci, which resulted in the juxtaposition of the MUM1 to the IgH 3' alpha-enhancer region by virtue of t(6;14) or insertion of the IgH sequences into the vicinity of the MUM1 gene and in the concomitant overexpression of the MUM1 mRNA. With similar results, fusions between MUM1 and IgH loci were observed by means of interphase DCFISH in eight (21.1%) out of the 38 MM cases, although no definite relationships between MUM1 status and specific clinical findings could be established.

引用

页码：1812 / 1816

页数：5