Detection of MUM1/IRF4-IgH fusion in multiple myeloma

被引：56
作者：
Yoshida, S
Nakazawa, N
Iida, S
Hayami, Y
Sato, S
Wakita, A
Shimizu, S
Taniwaki, M
Ueda, R
机构：
[1] Nagoya City Univ, Sch Med, Dept Internal Med 2, Mizuho Ku, Nagoya, Aichi 4678601, Japan
[2] Kyoto Prefectural Univ Med, Dept Internal Med 3, Kyoto 602, Japan
[3] Shimane Prefectural Cent Hosp, Izumo, Shimane, Japan
来源：
LEUKEMIA | 1999年 / 13卷 / 11期
关键词：
MUM1/IRF4; multiple myeloma; t(6; 14)(p25; q32); IgH insertion; FISH; PFGE;
D O I：
10.1038/sj.leu.2401563
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
MUM1 (multiple myeloma oncogene 1)/IRF4 (interferon regulatory factor 4) gene has been identified as an oncogene transcriptionally activated by t(6;14)(p25;q32) chromosomal translocation in multiple myeloma (MFA). The significance of this alteration in MM remains unknown, as it is not detectable by means of conventional cytogenetic analysis. To address this issue, we established diagnostic procedures based on pulsed-field gel electrophoresis (PFGE) analysis and double color fluorescence in site hybridization (DCFISH) using DNA probes derived from the MUM1 and the immunoglobulin heavy chain (IgH) gene loci. Among a panel of 17 MM cell lines, three (17.6%) showed fusions between these two loci, which resulted in the juxtaposition of the MUM1 to the IgH 3' alpha-enhancer region by virtue of t(6;14) or insertion of the IgH sequences into the vicinity of the MUM1 gene and in the concomitant overexpression of the MUM1 mRNA. With similar results, fusions between MUM1 and IgH loci were observed by means of interphase DCFISH in eight (21.1%) out of the 38 MM cases, although no definite relationships between MUM1 status and specific clinical findings could be established.
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页码：1812 / 1816
页数：5
相关论文
共 22 条
[1]   Promiscuous translocations into immunoglobulin heavy chain switch regions in multiple myeloma [J].
Bergsagel, PL ;
Chesi, M ;
Nardini, E ;
Brents, LA ;
Kirby, SL ;
Kuehl, WM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (24) :13931-13936
[2]   Dysregulation of cyclin D1 by translocation into an IgH gamma switch region in two multiple myeloma cell lines [J].
Chesi, M ;
Bergsagel, PL ;
Brents, LA ;
Smith, CM ;
Gerhard, DS ;
Kuehl, WM .
BLOOD, 1996, 88 (02) :674-681
[3]   The t(4;14) translocation in myeloma dysregulates both FGFR3 and a novel gene, MMSET, resulting in IgH/MMSET hybrid transcripts [J].
Chesi, M ;
Nardini, E ;
Lim, RSC ;
Smith, KD ;
Kuehl, WM ;
Bergsagel, PL .
BLOOD, 1998, 92 (09) :3025-3034
[4]   Frequent translocation t(4;14)(p16.3;q32.3) in multiple myeloma is associated with increased expression and activating mutations of fibroblast growth factor receptor 3 [J].
Chesi, M ;
Nardini, E ;
Brents, LA ;
Schrock, E ;
Ried, T ;
Kuehl, WM ;
Bergsagel, PL .
NATURE GENETICS, 1997, 16 (03) :260-264
[5]   Frequent dysregulation of the c-maf proto-oncogene at 16q23 by translocation to an Ig locus in multiple myeloma [J].
Chesi, M ;
Bergsagel, PL ;
Shonukan, OO ;
Martelli, ML ;
Brents, LA ;
Chen, T ;
Schröck, E ;
Ried, T ;
Kuehl, VM .
BLOOD, 1998, 91 (12) :4457-4463
[6]  
CORRADINI P, 1994, LEUKEMIA, V8, P758
[7]   Multiple myeloma: Increasing evidence for a multistep transformation process [J].
Hallek, M ;
Bergsagel, PL ;
Anderson, KC .
BLOOD, 1998, 91 (01) :3-21
[8]   Deregulation of MUM1/IRF4 by chromosomal translocation in multiple myeloma [J].
Iida, S ;
Rao, PH ;
Butler, M ;
Corradini, P ;
Boccadoro, M ;
Klein, B ;
Chaganti, RSK ;
DallaFavera, R .
NATURE GENETICS, 1997, 17 (02) :226-230
[9]  
IIDA S, 1997, REV CLIN EXP HEMATOL, V3, P60
[10]  
Lida S, 1996, BLOOD, V88, P4110
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