A Third-Generation Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Covalently Immobilized on Electrografted Organic Film on Screen-Printed Carbon Electrode

被引：22

作者：

Radi, Abd-Elgawad ^{[1
]}

Munoz-Berbel, Xavier ^{[2
]}

Cortina-Puig, Montserrat ^{[3
]}

Marty, Jean-Louis ^{[3
]}

机构：

[1] Mansoura Univ, Fac Sci, Dept Chem, Dumyat 34517, Egypt

[2] Esfera UAB, IMB CSIC, Ctr Nacl Microelect, Barcelona 08193, Spain

[3] Univ Perpignan, BIOMEM, F-66860 Perpignan, France

来源：

ELECTROANALYSIS | 2009年 / 21卷 / 14期

关键词：

Diazonium salt; Horseradish peroxidase; Direct electrochemistry; Hydrogen peroxide; Screen-printed carbon electrode; Biosensors; Enzymes; ELECTROCHEMICAL REDUCTION; AMBERLITE IRA-743; ENZYME SENSORS; OXIDATION; ANTHRAQUINONE; SURFACES; OXYGEN; HRP;

D O I：

10.1002/elan.200904587

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new convenient strategy to fabricate a third-generation hydrogen peroxide biosensor was described. The screen-printed carbon electrode (SPCE) was first modified with a layer of 4-nitrophenyl assembled from the 4-nitroaniline diazonium salt synthesized in situ in acidic aqueous solution. Next, the nitro groups were converted to amines followed by crosslinking to the horseradish peroxidase (HRP) by glutaraldehyde. The redox chemistry of the active center of the HRP was observed and the HRP-modified electrode displayed electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2) without any mediators. H2O2 was determined in a linear range from 5.0 mu M to 50.0 mu M, with a detection limit of 1.0 mu M. Furthermore, the biosensor exhibited fast amperometric response, good reproducibility and long-term stability.

引用

页码：1624 / 1629

页数：6

共 43 条

[31] Peroxidase-modified electrodes: Fundamentals and application [J].