Mutational analysis of primary alcohol metabolism in the methylotrophic actinomycete Amycolatopsis methanolica

被引：8
作者：
Hektor, HJ [1 ]
Dijkhuizen, L [1 ]
机构：
[1] UNIV GRONINGEN,DEPT MICROBIOL,GRONINGEN BIOMOL SCI & BIOTECHNOL INST,NL-9751 NN HAREN,NETHERLANDS
来源：
FEMS MICROBIOLOGY LETTERS | 1996年 / 144卷 / 01期
关键词：
actinomycete; Amycolatopsis methanolica; primary alcohol; ethanol; methanol; mutant; mutagenesis;
D O I：
10.1016/0378-1097(96)00341-2
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Mutants of the methylotrophic actinomycete Amycolatopsis methanolica unable to grow on methanol as carbon source were isolated and characterized. Mutants specifically affected in methanol utilization were deficient in formaldehyde assimilation. Mutants blocked in the first step of primary alcohol oxidation (C1-C4) had lost activity of the tetrazolium-dependent alcohol dehydrogenase, a three-component enzyme complex. This complex, or individual components, thus play a crucial role in utilization of primary alcohols in A. methanolica.
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收藏
页码：73 / 79
页数：7
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