Circulating T cell-monocyte complexes are markers of immune perturbations

被引：67

作者：

Burel, Julie G. ^{[1
]}

Pomaznoy, Mikhail ^{[1
]}

Arlehamn, Cecilia S. Lindestam ^{[1
]}

Weiskopf, Daniela ^{[1
]}

Antunes, Ricardo da Silva ^{[1
]}

Jung, Yunmin ^{[2
]}

Babor, Mariana ^{[1
]}

Schulten, Veronique ^{[1
]}

Seumois, Gregory ^{[1
]}

Greenbaum, Jason A. ^{[3
]}

Premawansa, Sunil ^{[4
]}

Premawansa, Gayani ^{[5
]}

Wijewickrama, Ananda ^{[6
]}

Vidanagama, Dhammika ^{[7
]}

Gunasena, Bandu ^{[8
]}

Tippalagama, Rashmi ^{[9
,16
]}

DeSilva, Aruna D. ^{[1
,9
,16
]}

Gilman, Robert H. ^{[10
,11
]}

Saito, Mayuko ^{[12
]}

Taplitz, Randy ^{[13
]}

Ley, Klaus ^{[2
,14
]}

Vijayanand, Pandurangan ^{[1
,15
]}

Sette, Alessandro ^{[1
,15
]}

Peters, Bjoern ^{[1
,15
]}

机构：

[1] La Jolla Inst Immunol, Div Vaccine Discovery, La Jolla, CA 92037 USA

[2] La Jolla Inst Immunol, Div Inflammat Biol, La Jolla, CA USA

[3] La Jolla Inst Immunol, Bioinformat Core, La Jolla, CA USA

[4] Univ Colombo, Sci Fac, Dept Zool & Environm Sci, Colombo, Sri Lanka

[5] North Colombo Teaching Hosp, Ragama, Sri Lanka

[6] Natl Inst Infect Dis, Gothatuwa, Sri Lanka

[7] Natl TB Reference Lab, Welisara, Sri Lanka

[8] Natl Hosp Resp Dis, Welisara, Sri Lanka

[9] Genetech Res Inst, Colombo, Sri Lanka

[10] Johns Hopkins Sch Publ Hlth, Baltimore, MD USA

[11] Univ Peruana Cayetano Heredia, Lima, Peru

[12] Tohoku Univ, Dept Virol, Grad Sch Med, Sendai, Miyagi, Japan

[13] Univ Calif San Diego, Div Infect Dis & Global Publ Hlth, La Jolla, CA 92093 USA

[14] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA

[15] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA

[16] Gen Sir John Kotelawala Def Univ, Fac Med, Dept Paraclin Sci, Ratmalana, Sri Lanka

来源：

ELIFE | 2019年 / 8卷

基金：

美国国家卫生研究院;

关键词：

HUMAN PERIPHERAL-BLOOD; MONONUCLEAR-CELLS; DIVERSITY; ACTIVATION; INFECTION; PROFILE; TARGET;

D O I：

10.7554/eLife.46045

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Our results highlight for the first time that a significant proportion of cell doublets in flow cytometry, previously believed to be the result of technical artifacts and thus ignored in data acquisition and analysis, are the result of biological interaction between immune cells. In particular, we show that cell: cell doublets pairing a T cell and a monocyte can be directly isolated from human blood, and high resolution microscopy shows polarized distribution of LFA1/ICAM1 in many doublets, suggesting in vivo formation. Intriguingly, T cell-monocyte complex frequency and phenotype fluctuate with the onset of immune perturbations such as infection or immunization, reflecting expected polarization of immune responses. Overall these data suggest that cell doublets reflecting T cell-monocyte in vivo immune interactions can be detected in human blood and that the common approach in flow cytometry to avoid studying cell: cell complexes should be re-visited.

引用

页数：21

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