Effect of CO2 Flow Rate on the Extraction of Astaxanthin and Fatty Acids from Haematococcus pluvialis Using Supercritical Fluid Technology

被引：25

作者：

Espinosa Alvarez, Carolina ^{[1
]}

Vardanega, Renata ^{[2
,3
]}

Salinas-Fuentes, Francisca ^{[1
]}

Palma Ramirez, Jenifer ^{[1
]}

Bugueno Munoz, Waldo ^{[1
]}

Jimenez-Rondon, Diana ^{[1
]}

Meireles, M. Angela A. ^{[2
]}

Cerezal Mezquita, Pedro ^{[1
]}

Ruiz-Dominguez, Mari Carmen ^{[1
]}

机构：

[1] Univ Antofagasta, Lab Microencapsulac Compuestos Bioactivos LAMICBA, Dept Ciencias Alimentos & Nutr, Fac Ciencias Salud FACSA, Avda Univ Antofagasta 02800,Campus Coloso, Antofagasta, Chile

[2] Univ Estadual Campinas, LASEFI Dept Food Engn, Sch Food Engn, UNICAMP, R Monteiro Lobato 80, BR-13083862 Campinas, SP, Brazil

[3] Entourage Phytolab, R Tabapua 111, BR-04533010 Sao Paulo, SP, Brazil

来源：

MOLECULES | 2020年 / 25卷 / 24期

关键词：

astaxanthin; supercritical fluid extraction; microalgae; fatty acids; CARBON-DIOXIDE EXTRACTION; HIGH-VALUE PRODUCTS; BIOACTIVE COMPOUNDS; VEGETABLE-OILS; PARTICLE-SIZE; MICROALGAE; CAROTENOIDS; PRESSURE; PURIFICATION; STABILITY;

D O I：

10.3390/molecules25246044

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Haematococcus pluvialis is the largest producer of natural astaxanthin in the world. Astaxanthin is a bioactive compound used in food, feed, nutraceutics, and cosmetics. In this study, astaxanthin extraction from H. pluvialis by supercritical fluid extraction was evaluated. The effects of temperature (40 and 50 degrees C), pressure (40 and 50 MPa), and CO2 flow rate (2 and 4 L/min) were investigated. The results showed that the highest astaxanthin recovery was obtained at 50 degrees C/50 MPa and the CO2 flow rates evaluated had no significant effect. It was possible to achieve astaxanthin recoveries of 95% after 175 min for a CO2 flow rate of 2 L/min, and 95 min for CO2 flow rate of 4 L/min. The omega-6/omega-3 ratios obtained were similar in all conditions, reaching 0.87, demonstrating that the extracts from H. pluvialis by SFE are rich in unsaturated fatty acids (UFA) which increases their positive effects when used as a functional ingredient in food.

引用

页数：17

共 65 条

[21] Supercritical Carbon Dioxide Extraction of Astaxanthin, Lutein, and Fatty Acids from Haematococcus pluvialis Microalgae [J].

Di Sanzo, Giuseppe ;

Mehariya, Sanjeet ;

Martino, Maria ;

Larocca, Vincenzo ;

Casella, Patrizia ;

Chianese, Simeone ;

Musmarra, Dino ;

Balducchi, Roberto ;

Molino, Antonio .

MARINE DRUGS, 2018, 16 (09)

[22] Isolation of astaxanthin monoesters from the microalgae Haematococcus pluvialis by high performance countercurrent chromatography (HPCCC) combined with high performance liquid chromatography (HPLC) [J].

Fabryova, Tereza ;

Tumova, Lenka ;

da Silva, Daniela Correia ;

Pereira, David M. ;

Andrade, Paula B. ;

Valentao, Patricia ;

Hrouzek, Pavel ;

Kopecky, Jiri ;

Cheel, Jose .

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 2020, 49

[23] Changes of carotenoids contents and analysis of astaxanthin geometrical isomerization in Haematococcus pluvialis under outdoor high light conditions [J].

Gong, Fengying ;

Zhang, Chunhui ;

Zhang, Litao ;

Liu, Jianguo .

AQUACULTURE RESEARCH, 2020, 51 (02) :770-778

[24] Carotenoids from microalgae: A review of recent developments [J].

Gong, Mengyue ;

Bassi, Amarjeet .

BIOTECHNOLOGY ADVANCES, 2016, 34 (08) :1396-1412

[25] Ultrastructural and chemical changes in the cell wall of Haematococcus pluvialis (Volvocales, Chlorophyta) during aplanospore formation [J].

Hagen, C ;

Siegmund, S ;

Braune, W .

EUROPEAN JOURNAL OF PHYCOLOGY, 2002, 37 (02) :217-226

[26] Determination of astaxanthin and astaxanthin esters in the microalgae Haematococcus pluvialis by LC-(APCI)MS and characterization of predominant carotenoid isomers by NMR spectroscopy [J].

Holtin, Karsten ;

Kuehnle, Maximilian ;

Rehbein, Jens ;

Schuler, Paul ;

Nicholson, Graeme ;

Albert, Klaus .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2009, 395 (06) :1613-1622

[27]

Jesus S. P., 2013, Food and Public Health, V3, P215

[28] SC-CO2 extraction of Vitex agnus-castus L. fruits: The influence of pressure, temperature and water presoaking on the yield and GC-MS profiles of the extracts in comparison to the essential oil composition [J].

Jokic, Stela ;

Jerkovic, Igor ;

Rajic, Marina ;

Aladic, Krunoslav ;

Bilic, Mate ;

Vidovic, Senka .

JOURNAL OF SUPERCRITICAL FLUIDS, 2017, 123 :50-57

[29] Microalgae as versatile cellular factories for valued products [J].

Koller, Martin ;

Muhr, Alexander ;

Braunegg, Gerhart .

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 2014, 6 :52-63

[30] Supercritical carbon dioxide extraction of astaxanthin from Haematococcus pluvialis with vegetable oils as co-solvent [J].

Krichnavaruk, Sontaya ;

Shotipruk, Artiwan ;

Goto, Motonobu ;

Pavasant, Prasert .

BIORESOURCE TECHNOLOGY, 2008, 99 (13) :5556-5560

← 1 2 3 4 5 6 7 →