Effect of CO2 Flow Rate on the Extraction of Astaxanthin and Fatty Acids from Haematococcus pluvialis Using Supercritical Fluid Technology

被引:25
作者
Espinosa Alvarez, Carolina [1 ]
Vardanega, Renata [2 ,3 ]
Salinas-Fuentes, Francisca [1 ]
Palma Ramirez, Jenifer [1 ]
Bugueno Munoz, Waldo [1 ]
Jimenez-Rondon, Diana [1 ]
Meireles, M. Angela A. [2 ]
Cerezal Mezquita, Pedro [1 ]
Ruiz-Dominguez, Mari Carmen [1 ]
机构
[1] Univ Antofagasta, Lab Microencapsulac Compuestos Bioactivos LAMICBA, Dept Ciencias Alimentos & Nutr, Fac Ciencias Salud FACSA, Avda Univ Antofagasta 02800,Campus Coloso, Antofagasta, Chile
[2] Univ Estadual Campinas, LASEFI Dept Food Engn, Sch Food Engn, UNICAMP, R Monteiro Lobato 80, BR-13083862 Campinas, SP, Brazil
[3] Entourage Phytolab, R Tabapua 111, BR-04533010 Sao Paulo, SP, Brazil
关键词
astaxanthin; supercritical fluid extraction; microalgae; fatty acids; CARBON-DIOXIDE EXTRACTION; HIGH-VALUE PRODUCTS; BIOACTIVE COMPOUNDS; VEGETABLE-OILS; PARTICLE-SIZE; MICROALGAE; CAROTENOIDS; PRESSURE; PURIFICATION; STABILITY;
D O I
10.3390/molecules25246044
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Haematococcus pluvialis is the largest producer of natural astaxanthin in the world. Astaxanthin is a bioactive compound used in food, feed, nutraceutics, and cosmetics. In this study, astaxanthin extraction from H. pluvialis by supercritical fluid extraction was evaluated. The effects of temperature (40 and 50 degrees C), pressure (40 and 50 MPa), and CO2 flow rate (2 and 4 L/min) were investigated. The results showed that the highest astaxanthin recovery was obtained at 50 degrees C/50 MPa and the CO2 flow rates evaluated had no significant effect. It was possible to achieve astaxanthin recoveries of 95% after 175 min for a CO2 flow rate of 2 L/min, and 95 min for CO2 flow rate of 4 L/min. The omega-6/omega-3 ratios obtained were similar in all conditions, reaching 0.87, demonstrating that the extracts from H. pluvialis by SFE are rich in unsaturated fatty acids (UFA) which increases their positive effects when used as a functional ingredient in food.
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页数:17
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