Extremely low frequency pulsed DC electric fields promote neutrophil extension, metabolic resonance and DNA damage when phase-matched with metabolic oscillators

被引:37
作者
Kindzelskii, AL [1 ]
Petty, HR [1 ]
机构
[1] Wayne State Univ, Dept Biol Sci, Detroit, MI 48202 USA
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 2000年 / 1495卷 / 01期
基金
美国国家卫生研究院;
关键词
neutrophil; electric field; cytoskeleton; biophysical phase relationship; metabolism; signal processing; oscillation;
D O I
10.1016/S0167-4889(99)00148-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Application of extremely low frequency pursed DC electric fields that are frequency- and phase-matched with endogenous metabolic oscillations leads to greatly exaggerated neutrophil extension and metabolic resonance wherein oscillatory NAD(P)H amplitudes are increased. In the presence of a resonant field, migrating cell length grows from 10 to approximate to 40 mu m, as does the overall length of microfilament assemblies. in contrast, cells stop locomotion and become spherical when exposed to phase-mismatched fields. Although cellular effects were not found to be dependent on electrode type and buffer, they were sensitive to temporal constraints (phase and pulse length) and cell surface charge. We suggest an electromechanical coupling hypothesis wherein applied electric fields and cytoskeletal polymerization forces act together to overcome the surface/cortical tension of neutrophils, thus promoting net cytoskeletal assembly and heightened metabolic amplitudes. Metabolic resonance enhances reactive oxygen metabolic production by neutrophils. Furthermore, cellular DNA damage was observed after prolonged metabolic resonance using both single cell gel electrophoresis ('comet' assay) and 3'-OH DNA labeling using terminal deoxynucleotidyl transferase. These results provide insights into transmembrane signal processing and cell interactions with weak electric fields. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:90 / 111
页数:22
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