Wnt-1 Protein as a Prognostic Biomarker for Hepatitis B-Related and Hepatitis C-Related Hepatocellular Carcinoma after Surgery

被引：29

作者：

Lee, Hao-Hsien

Uen, Yih-Huei ^{[1
]}

Tian, Yu-Feng ^{[1
]}

Sun, Chi-Shu ^{[2
]}

Sheu, Ming-Jen ^{[2
]}

Kuo, Hsing-Tao ^{[2
]}

Koay, Lok-Beng ^{[2
]}

Lin, Ching-Yih ^{[2
]}

Tzeng, Ching-Cherng ^{[3
]}

Cheng, Chia-Ju ^{[4
]}

Tang, Ling-Yu ^{[4
]}

Tsai, Sun-Lung ^{[2
,4
]}

Wang, Andrew H-J. ^{[5
,6
]}

机构：

[1] Chi Mei Med Ctr, Dept Gen Surg, Tainan, Taiwan

[2] Chi Mei Med Ctr, Dept Internal Med, Tainan, Taiwan

[3] Chi Mei Med Ctr, Dept Pathol, Tainan, Taiwan

[4] Chi Mei Med Ctr, Dept Med Res, Tainan, Taiwan

[5] Acad Sinica, Inst Biol Chem, Taipei, Taiwan

[6] Acad Sinica, Core Facil Prote Res, Taipei, Taiwan

来源：

CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION | 2009年 / 18卷 / 05期

关键词：

BETA-CATENIN MUTATIONS; NF-KAPPA-B; INTRAHEPATIC RECURRENCE; GENE-EXPRESSION; E-CADHERIN; VIRUS; PATHWAY; ACTIVATION; HEPATOCARCINOGENESIS; PATHOGENESIS;

D O I：

10.1158/1055-9965.EPI-09-0039

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Up-regulation of Wnt-1 protein has been reported in hepatitis B virus (HBV)-related and hepatitis C virus (HCV)-related hepatocellular carcinoma (HCC) tissues and cell lines. It is known to play a fundamental role in signaling cancer progression, whereas its prognostic role in HCC remains unexplored. Methods: As a prognostic biomarker, this study analyzed Wnt-1 protein expression in 63 histology-verified HCC patients receiving curative resection. In each paired tumor and nontumor specimen, Wnt-1 levels were semi quantitatively measured by Western blotting and expressed by tumor/nontumor ratio. The data were further correlated with quantitative real-time PCR as well as with beta-catenin and E-cadherin expression by immunohistochemistry. Cumulative tumor recurrence-free survival curves were constructed using the Kaplan-Meier method and compared by the log-rank test. Results: The results showed that 26 (group 1) and 37 (group 11) HCC patients had an expression ratio of Wnt-1 >= 1.5 and <1.5, respectively. The amount of Wnt-1 estimated by tumor/nontumor ratio correlated with the results by quantitative real-time PCR. High tumor Wnt-1 expression correlated with enhanced nuclear beta-catenin accumulation, diminished membranous E-cadherin expression, and increased tumor recurrence after curative tumor resection. Conclusions: These results suggest that Wnt-1 may be used as a predisposing risk factor for HCC recurrence. The use of tumor Wnt-1 as prognostic biomarker may identify patients with HBV- and/or HCV-related HCC patients with a high risk of tumor recurrence who may then benefit from further intensive therapy after surgery. (Cancer Epidemiol Biomarkers Prev 2009;18(5):1562-9)

引用

页码：1562 / 1569

页数：8

共 56 条

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