Chronic Ethanol Exposure during Adolescence in Rats Induces Motor Impairments and Cerebral Cortex Damage Associated with Oxidative Stress

被引:55
作者
Teixeira, Francisco Bruno [1 ]
da Silva Santana, Luana Nazare [1 ]
Bezerra, Fernando Romualdo [2 ]
De Carvalho, Sabrina [2 ]
Fontes-Junior, Eneas Andrade [2 ,3 ]
Prediger, Rui Daniel [4 ]
Crespo-Lopez, Maria Elena [3 ]
Ferraz Maia, Cristiane Socorro [2 ]
Lima, Rafael Rodrigues [1 ]
机构
[1] Fed Univ Para, Inst Biol Sci, Lab Funct & Struct Biol, BR-66059 Belem, Para, Brazil
[2] Fed Univ Para, Inst Hlth Sci, Lab Pharmacol Inflammat & Behav, BR-66059 Belem, Para, Brazil
[3] Fed Univ Para, Inst Biol Sci, Mol Pharmacol Lab, BR-66059 Belem, Para, Brazil
[4] Univ Fed Santa Catarina, Ctr Biol Sci, Dept Pharmacol, Florianopolis, SC, Brazil
来源
PLOS ONE | 2014年 / 9卷 / 06期
关键词
NF-KAPPA-B; ALCOHOL EXPOSURE; INFLAMMATORY MEDIATORS; COGNITIVE DEFICITS; PROTECT NEURONS; CELL-DEATH; ADULT RATS; BRAIN; MICROGLIA; HIPPOCAMPUS;
D O I
10.1371/journal.pone.0101074
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Binge drinking is common among adolescents, and this type of ethanol exposure may lead to long-term nervous system damage. In the current study, we evaluated motor performance and tissue alterations in the cerebral cortex of rats subjected to intermittent intoxication with ethanol from adolescence to adulthood. Adolescent male Wistar rats (35 days old) were treated with distilled water or ethanol (6.5 g/kg/day, 22.5%w/v) during 55 days by gavage to complete 90 days of age. The open field, inclined plane and the rotarod tests were used to assess the spontaneous locomotor activity and motor coordination performance in adult animals. Following completion of behavioral tests, half of animals were submitted to immunohistochemical evaluation of NeuN (marker of neuronal bodies), GFAP (a marker of astrocytes) and Iba1 (microglia marker) in the cerebral cortex while the other half of the animals were subjected to analysis of oxidative stress markers by biochemical assays. Chronic ethanol intoxication in rats from adolescence to adulthood induced significant motor deficits including impaired spontaneous locomotion, coordination and muscle strength. These behavioral impairments were accompanied by marked changes in all cellular populations evaluated as well as increased levels of nitrite and lipid peroxidation in the cerebral cortex. These findings indicate that continuous ethanol intoxication from adolescence to adulthood is able to provide neurobehavioral and neurodegenerative damage to cerebral cortex.
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页数:8
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