Immobilization of horseradish peroxidase on zinc oxide nanorods grown directly on electrodes for hydrogen peroxide sensing

被引:10
作者
Zhang, Wen [1 ]
Guo, Cong [1 ]
Chang, Yingxing [1 ]
Wu, Fengyan [1 ]
Ding, Shujiang [1 ]
机构
[1] Xi An Jiao Tong Univ, Sch Sci, Xian 710049, Peoples R China
来源
MONATSHEFTE FUR CHEMIE | 2014年 / 145卷 / 01期
关键词
Nanostructures; Electrochemisty; Biosensors; Enzymes; Electron transfer; ZNO-NANOWIRE ARRAY; DIRECT ELECTROCHEMISTRY; GLUCOSE-OXIDASE; BIOSENSOR; FABRICATION; TIME;
D O I
10.1007/s00706-013-0979-6
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
An amperometric biosensor for hydrogen peroxide detection was developed by immobilization of horseradish peroxidase on zinc oxide nanorods directly grown on the electrode. The ZnO nanorods were fabricated by electrodepositing ZnO nanoseeds on indium tin oxide glass followed by a hydrothermal growth process. The ZnO nanorods were used to increase the specific surface area for carrying horseradish peroxidase and to build direct electron transfer channels to the electrode. The structure of the ZnO nanorods on indium tin oxide was studied by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and the electrochemical behaviors were carefully examined. The result showed that the biosensor responds well to hydrogen peroxide in a wide range of concentration. The linear range is from 0.5 to 9.0 mM with a limit of detection (S/N = 3) of 0.3 mM, and the response time is about 7 s, which indicates that ZnO nanorods are promising supporting materials to build direct electron transfer biosensors.
引用
收藏
页码:107 / 112
页数:6
相关论文
共 27 条
[11]   Synthesis of photoconducting ZnO nano-needles using an unbalanced magnetron sputtered ZnO/Zn/ZnO multilayer structure [J].
Kumar, S ;
Gupta, V ;
Sreenivas, K .
NANOTECHNOLOGY, 2005, 16 (08) :1167-1171
[12]   Fabrication of hydrogen peroxide biosensor based on Ni doped SnO2 nanoparticles [J].
Lavanya, N. ;
Radhakrishnan, S. ;
Sekar, C. .
BIOSENSORS & BIOELECTRONICS, 2012, 36 (01) :41-47
[13]   High intensity, plasma-induced emission from large area ZnO nanorod array cathodes [J].
Liao, Qingliang ;
Yang, Ya ;
Xia, Liansheng ;
Qi, Junjie ;
Zhang, Yue ;
Huang, Yunhua ;
Qin, Zi .
PHYSICS OF PLASMAS, 2008, 15 (11)
[14]   Carbon-decorated ZnO nanowire array: A novel platform for direct electrochemistry of enzymes and biosensing applications [J].
Liu, Jinping ;
Guo, Chunxian ;
Li, Chang Ming ;
Li, Yuanyuan ;
Chi, Qingbo ;
Huang, Xintang ;
Liao, Lei ;
Yu, Ting .
ELECTROCHEMISTRY COMMUNICATIONS, 2009, 11 (01) :202-205
[15]   Aligned ZnO nanorods: A useful film to fabricate amperometric glucose biosensor [J].
Liu, Xiaowang ;
Hu, Qiyan ;
Wu, Qiong ;
Zhang, Wei ;
Fang, Zhen ;
Xie, Qiubo .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2009, 74 (01) :154-158
[16]   TIME-DEPENDENT MICHAELIS-MENTEN KINETICS FOR AN ENZYME-SUBSTRATE-INHIBITOR SYSTEM [J].
RUBINOW, SI ;
LEBOWITZ, JL .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1970, 92 (13) :3888-&
[17]   Complex and oriented ZnO nanostructures [J].
Tian, ZRR ;
Voigt, JA ;
Liu, J ;
McKenzie, B ;
McDermott, MJ ;
Rodriguez, MA ;
Konishi, H ;
Xu, HF .
NATURE MATERIALS, 2003, 2 (12) :821-826
[18]   ZnO Nanorods Based Hydrazine Sensors [J].
Umar, Ahmad ;
Rahman, M. M. ;
Hahn, Yoon-Bong .
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2009, 9 (08) :4686-4691
[19]   Growth of arrayed nanorods and nanowires of ZnO from aqueous solutions [J].
Vayssieres, L .
ADVANCED MATERIALS, 2003, 15 (05) :464-466
[20]   Zinc oxide nanocomb biosensor for glucose detection [J].
Wang, J. X. ;
Sun, X. W. ;
Wei, A. ;
Lei, Y. ;
Cai, X. P. ;
Li, C. M. ;
Dong, Z. L. .
APPLIED PHYSICS LETTERS, 2006, 88 (23)