Effect of Gallic acid in potentiating chemotherapeutic effect of Paclitaxel in HeLa cervical cancer cells

被引：71

作者：

Aborehab, Nora M. ^{[1
]}

Osama, Nada ^{[2
]}

机构：

[1] October Univ Modern Sci & Arts, MSA Univ, Fac Pharm, Dept Biochem, Giza 12611, Egypt

[2] Menoufia Univ, Fac Pharm, Dept Biochem, Menoufia 32511, Egypt

来源：

CANCER CELL INTERNATIONAL | 2019年 / 19卷 / 1期

关键词：

HeLa cells; Gallic acid; Paclitaxel; P53; Caspase; 3; NATURAL-PRODUCTS; BREAST-CANCER; CYCLE ARREST; CISPLATIN; APOPTOSIS; CARBOPLATIN; GROWTH; DEATH; ERK; PROLIFERATION;

D O I：

10.1186/s12935-019-0868-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

BackgroundCervical cancer is the fourth most common cancer affecting women worldwide. Paclitaxel/Carboplatin is one of the most commonly prescribed regimens in cervical cancer treatment. Although chemotherapeutic drugs are very effective, severe side effects and development of drug resistance limits the use of these drugs. The use of natural products with anticancer activity may help to partially overcome these issues.MethodsIn the present study, we investigated the ability of Gallic acid, to potentiate the anti-cancer effects of Paclitaxel, Carboplatin and Paclitaxel/Carboplatin combination in human HeLa cells by performing MTT assay, cell cycle analysis and RT-PCR assay and Western blotting for some apoptotic markers.ResultsOur results revealed that the highest cytotoxic effect, the highest induction of apoptosis and significant elevation in P53 and Caspase 3 levels was seen in Paclitaxel/Gallic acid combination.ConclusionThese results indicate that Gallic acid potentiates Paclitaxel effect and that Paclitaxel/Gallic acid combination could represent a promising alternative with lower side effects-for Paclitaxel/Carboplatin combination in treatment of cervical cancer treatment.

引用

页数：13

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