Structural elucidation and cellular antioxidant activity evaluation of major antioxidant phenolics in lychee pulp

被引：63

作者：

Su, Dongxiao ^{[1
,2
]}

Ti, Huihui ^{[1
]}

Zhang, Ruifen ^{[1
]}

Zhang, Mingwei ^{[1
]}

Wei, Zhengchen ^{[1
]}

Deng, Yuanyuan ^{[1
]}

Guo, Jinxin ^{[1
]}

机构：

[1] Guangdong Acad Agr Sci, Minist Agr, Key Lab Funct Foods, Sericultural & Agri Food Res Inst, Guangzhou 510610, Guangdong, Peoples R China

[2] Huazhong Agr Univ, Dept Food Sci & Technol, Wuhan 430070, Peoples R China

来源：

FOOD CHEMISTRY | 2014年 / 158卷

基金：

对外科技合作项目（国际科技项目）;

关键词：

Lychee; Phenolics; Quercetin; 3-0-rutinoside-7-0-alpha-L-rhamnosidase; Antioxidant activity; Cellular antioxidant activity; Oxygen radical absorbance capacity; LITCHI-CHINENSIS; IDENTIFICATION; FLAVONOIDS; PERICARP; QUERCETIN-3-GLUCOSIDE; METABOLISM; ABSORPTION; MEMBRANE; PROFILES; EXTRACTS;

D O I：

10.1016/j.foodchem.2014.02.134

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Lychee pulp contains phenolic compounds that are strong antioxidants, but the identities of the major antioxidants present are unknown. In the present study, the major contributors to the antioxidant activity of fresh lychee pulp were identified and their cellular antioxidant activities were investigated. Aqueous acetone extracts of lychee pulp were fractionated on polyamide resin, and those fractions with the largest antioxidant and radical scavenging activities were selected using cellular antioxidant activity and oxygen radical absorbance capacity assays. Three compounds that were major contributors to the antioxidant activity in these fractions were obtained by reverse-phase preparative HPLC and identified as quercetin 3-0-rutinoside-7-0-alpha-L-rhamnosidase (quercetin 3-rut-7-rha), quercetin 3-0-rutinoside (rutin) and ()-epicatechin using NMR spectroscopy, HMBC, and ESI-MS spectrometry. The concentration of quercetin 3-rut-7-rha was 17.25 mg per 100 g of lychee pulp fresh weight. This is the first report of the identification and cellular antioxidant activity of quercetin 3-rut-7-rha from lychee pulp. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：385 / 391

页数：7

共 35 条

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