Change in substrate preference of Streptomyces aminopeptidase through modification of the environment around the substrate binding site

被引：4

作者：

Arima, Jiro ^{[1
]}

Uesugi, Yoshiko ^{[1
]}

Iwabuchi, Masaki ^{[1
]}

Hatanaka, Tadashi ^{[1
]}

机构：

[1] RIBS, Okayama 7161241, Japan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 12期

关键词：

D O I：

10.1128/AEM.01460-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We attempted to alter the substrate preference of aminopeptidase from Streptomyces septatus TH-2 (SSAP). Because Asp198 and Phe221 of SSAP are located in the substrate binding site, we screened 2,000 mutant enzymes with D198X/F221X mutations. By carrying out this examination, we obtained two enzymes; one specifically hydrolyzed an arginyl derivative, and the other specifically hydrolyzed a cystinyl derivative (65- and 12.5-fold higher k(cat) values for hydrolysis of p-nitroanilide derivatives than those of the wild type, respectively).

引用

页码：7962 / 7967

页数：6

共 27 条

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