Monitoring antibody-antigen reactions at conducting polymer-based immunosensors using impedance spectroscopy

被引:81
作者
Sargent, A [1 ]
Sadik, OA [1 ]
机构
[1] SUNY Binghamton, Dept Chem, Binghamton, NY 13902 USA
关键词
mechanism; charge generation; charge propagation; antibody-antigen reactions; conducting polymers; immunochemical sensor;
D O I
10.1016/S0013-4686(99)00265-0
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
The mechanisms of antibody-antigen (Ab-Ag) interactions at conducting polypyrrole electrodes have been investigated using impedance spectroscopy techniques. The effects of the variation in ion exchange, solution composition, and the condition of the synthesis have been used to study the capacitive behavior of antibody-containing polypyrrole electrodes in the presence of the antigen. The theory of charge generation and transportation in the heterogeneous polymeric domains is proposed as the predominant basis for the analytical signals observed at these electrodes. The significant difference observed in the impedance response at different potentials confirmed that the Ab-Ag interaction was largely influenced by the applied potential. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
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页码:4667 / 4675
页数:9
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