Transection injury differentially alters the proteome of the human sural nerve

被引:4
作者
Chau, Monica J. [1 ,2 ]
Quintero, Jorge E. [1 ,2 ,3 ]
Blalock, Eric [4 ]
Byrum, Stephanie [5 ]
Mackintosh, Samuel G. [5 ]
Samaan, Christopher [1 ,2 ]
Gerhardt, Greg A. [1 ,2 ,3 ,6 ]
van Horne, Craig G. [1 ,2 ,3 ]
机构
[1] Univ Kentucky, Coll Med, Brain Restorat Ctr, Lexington, KY 40536 USA
[2] Univ Kentucky, Coll Med, Dept Neurosurg, Lexington, KY 40536 USA
[3] Univ Kentucky, Coll Med, Dept Neurosci, Lexington, KY 40536 USA
[4] Univ Kentucky, Coll Med, Dept Pharmacol & Nutr Sci, Lexington, KY USA
[5] Univ Arkansas Med Sci, Dept Biochem & Mol Biol, Little Rock, AR USA
[6] Univ Kentucky, Coll Med, Dept Neurol, Lexington, KY USA
关键词
IDIOPATHIC PARKINSONS-DISEASE; REPROGRAMS SCHWANN-CELLS; PERIPHERAL-NERVE; C-JUN; AXONAL REGENERATION; NEUROTROPHIC FACTOR; RETROSPECTIVE SURVEY; REPAIR; MOTOR; GDNF;
D O I
10.1371/journal.pone.0260998
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Regeneration after severe peripheral nerve injury is often poor. Knowledge of human nerve regeneration and the growth microenvironment is greatly lacking. We aimed to identify the regenerative proteins in human peripheral nerve by comparing the proteome before and after a transection injury. In a unique study design, we collected closely matched samples of naive and injured sural nerve. Naive and injured (two weeks after injury) samples were analyzed using mass spectrometry and immunoassays. We found significantly altered levels following the nerve injury. Mass spectrometry revealed that injury samples had 568 proteins significantly upregulated and 471 significantly downregulated compared to naive samples (q-value <= 0.05 and Z >= |2| (log2)). We used Gene Ontology (GO) pathway overrepresentation analysis to highlight groups of proteins that were significantly upregulated or downregulated with injury-induced degeneration and regeneration. Significant protein changes in key pathways were identified including growth factor levels, Schwann cell de-differentiation, myelination downregulation, epithelial-mesenchymal transition (EMT), and axonal regeneration pathways. The proteomes of the uninjured nerve compared to the degenerating/regenerating nerve may reveal biomarkers to aid in the development of repair strategies such as infusing supplemental trophic factors and in monitoring neural tissue regeneration.
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页数:24
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