CRISPR/Cas9-based genome engineering: A new breakthrough in the genetic manipulation of filamentous fungi

被引：30
作者：
Ullah, Mati [1 ]
Xia, Lin [1 ]
Xie, Shangxian [1 ]
Sun, Su [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Dept Biotechnol, Coll Life Sci & Technol, Wuhan, Hubei, Peoples R China
来源：
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2020年
基金：
中国国家自然科学基金;
关键词：
CRISPR; Cas9; filamentous fungi; genome engineering; AGROBACTERIUM-MEDIATED TRANSFORMATION; TANDEM SHOCK-WAVES; CRISPR-CAS; FUNCTIONAL GENOMICS; IN-VIVO; TRICHODERMA-REESEI; ASPERGILLUS-NIGER; SYSTEM; RNA; DNA;
D O I：
10.1002/bab.2077
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Filamentous fungi have several industrial, environmental, and medical applications. However, they are rarely utilized owing to the limited availability of full-genome sequences and genetic manipulation tools. Since the recent discovery of the full-genome sequences for certain industrially important filamentous fungi, CRISPR/Cas9 technology has drawn attention for the efficient development of engineered strains of filamentous fungi. CRISPR/Cas9 genome editing has been successfully applied to diverse filamentous fungi. In this review, we briefly discuss the use of common genetic transformation techniques as well as CRISPR/Cas9-based systems in filamentous fungi. Furthermore, we describe potential limitations and challenges in the practical application of genome engineering of filamentous fungi. Finally, we provide suggestions and highlight future research prospects in the area.
引用
收藏
页码：835 / 851
页数：17
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