Determination of Verapamil in Exhaled Breath Condensate by using Microextraction and Liquid Chromatography

被引：4

作者：

Pourkarim, Fariba ^{[1
,2
,3
]}

Shayanfar, Ali ^{[2
,4
]}

Khoubnasabjafari, Maryam ^{[5
]}

Akbarzadeh, Fariborz ^{[6
]}

Sajedi-Amin, Sanaz ^{[7
]}

Jouyban-Gharamaleki, Vahid ^{[7
,8
]}

Jouyban, Abolghasem ^{[1
,2
,8
]}

机构：

[1] Tabriz Univ Med Sci, Pharmaceut Anal Res Ctr, Tabriz, Iran

[2] Tabriz Univ Med Sci, Fac Pharm, Tabriz, Iran

[3] Tabriz Univ Med Sci, Student Res Comm, Tabriz, Iran

[4] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz 51664, Iran

[5] Tabriz Univ Med Sci, TB & Lung Dis Res Ctr, Tabriz, Iran

[6] Tabriz Univ Med Sci, Cardiovasc Res Ctr, Tabriz, Iran

[7] Tabriz Univ Med Sci, Liver & Gastrointestinal Dis Res Ctr, Tabriz, Iran

[8] Tabriz Univ Med Sci, Kimia Idea Pardaz Azarbayjan KIPA Sci Based Co, Tabriz, Iran

来源：

CURRENT PHARMACEUTICAL ANALYSIS | 2019年 / 15卷 / 05期

关键词：

Verapamil; dispersive liquid-liquid microextraction; high-performance liquid chromatography; exhaled breath condensate; cardiovascular diseases; mortality; HUMAN-PLASMA; ANTIARRHYTHMIC-DRUGS; MASS-SPECTROMETRY; HUMAN SERUM; NORVERAPAMIL; METHADONE; EXTRACTION; DELTA(9)-TETRAHYDROCANNABINOL; PHARMACOKINETICS; QUANTIFICATION;

D O I：

10.2174/1573412914666180717125434

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Background: Developing a simple analysis method for quantification of drug concentration is one of the essential issues in pharmacokinetic and therapeutic drug monitoring studies. Objective: A fast and reliable dispersive liquid-liquid microextraction procedure was employed for preconcentration of verapamil in exhaled breath condensate (EBC) samples and this was followed by the determination with high-performance liquid chromatography-ultraviolet detection. Methods: A reverse-phase high-performance liquid chromatography (RP-HPLC) combined with a dispersive liquid-liquid microextraction method (DLLME) was applied for quantification of verapamil in the EBC samples. The developed method was validated according to FDA guidelines. Results: Under the optimum conditions, the method provided a linear range between 0.07 and 0.8 mu g.mL(-1) with a coefficient of determination of 0.998. The intra- and inter-day relative standard deviation and relative error values of the method were below 15%, which indicated good precision and accuracy. The proposed method was successfully applied for the analysis of verapamil in two real samples with concentrations of 0.07 and 0.09 mu g.mL(-1). Conclusion: The established HPLC-UV-DLLME method could be applied for the analysis of verapamil in human EBC samples.

引用

页码：535 / 541

页数：7

共 49 条

[1] VERAPAMIL AND NORVERAPAMIL DETERMINATION IN HUMAN-PLASMA BY GAS-LIQUID-CHROMATOGRAPHY USING NITROGEN-PHOSPHORUS DETECTION - APPLICATION TO SINGLE-DOSE PHARMACOKINETIC STUDIES [J].