In vivo induction of proinflammatory cytokines in mouse tissue by Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans

被引:65
|
作者
Kesavalu, L
Chandrasekar, B
Ebersole, JL
机构
[1] Univ Kentucky, Coll Dent, Ctr Oral Hlth Res, Lexington, KY 40536 USA
[2] Univ Kentucky, Coll Dent, Dept Oral Hlth Sci, Lexington, KY 40536 USA
[3] Univ Texas, Hlth Sci Ctr, Cardiol Div, Dept Med, San Antonio, TX USA
来源
ORAL MICROBIOLOGY AND IMMUNOLOGY | 2002年 / 17卷 / 03期
关键词
Actinobacillus actinomycetemcomitans; calvaria; cytokines; Porphyromonas gingivalis; Streptococcus gordonii;
D O I
10.1034/j.1399-302X.2002.170307.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Periodontitis is a chronic inflammatory disease initiated by a multitude of bacteria. Persistent infection leads to generation of various inflammatory mediators, resulting in tissue destruction and osteoclastic resorption of the alveolar bone. This study describes a novel in vivo murine calvarial model to assess the effects of oral pathogens on the expression of three proinflammatory cytokines [interleukin (IL)-Ibeta, IL-6, and tumor necrosis factor (TNF)-alpha which are involved in bone resorption. We chose Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans as prototype oral pathogens. We also tested the effects of Streptococcus gordonii, an oral commensal supragingival microorganism, considered a non-pathogen. Live bacteria were injected into subcutaneous tissue overlying the parietal bone of mice calvaria for 6days. At the end of the experimental period, tissues overlying the calvaria were removed and analyzed for proinflammatory cytokine expression by Northern blotting. Cytokine mRNA was not detected in the tissue over the calvaria of control animals. In contrast, P. gingivalis and A. actinomycetemcomitans elicited mRNA expression of all three cytokines, TNFalpha being the highest (TNFalpha > >IL-1beta > IL-6). P. gingivalis was more potent than A. actinomycetemcomitans in inducing cytokine expression. In contrast, S. gordonii induced only low levels of mRNA for IL-1beta and TNFalpha but no IL-6 mRNA induction. These results suggest that oral microorganisms with access to host tissues elicit a battery of proinflammatory cytokines. There were clear differences in profiles and, interestingly, a commensal bacterium also stimulated bone resorptive cytokine expression in host tissues.
引用
收藏
页码:177 / 180
页数:4
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