Differentiation of human adipose-derived stem cells seeded on mineralized electrospun co-axial poly(ε-caprolactone) (PCL)/gelatin nanofibers

被引：47

作者：

Pereira, Ildeu H. L. ^{[1
]}

Ayres, Eliane ^{[2
]}

Averous, Luc ^{[3
]}

Schlatter, Guy ^{[3
]}

Hebraud, Anne ^{[3
]}

Chagas de Paula, Ana Claudia ^{[4
]}

Leroy Viana, Pedro Henrique ^{[4
]}

Goes, Alfredo Miranda ^{[4
]}

Orefice, Rodrigo L. ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Dept Met & Mat Engn, Belo Horizonte, MG, Brazil

[2] Minas Gerais State Univ UFMG, Sch Design, Dept Mat Technol & Proc, Belo Horizonte, MG, Brazil

[3] Univ Strasbourg, ICPEES ECPM, UMR 7515, F-67087 Strasbourg 2, France

[4] Univ Fed Minas Gerais, Dept Biochem & Imunol, Belo Horizonte, MG, Brazil

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2014年 / 25卷 / 04期

关键词：

CALCIUM-PHOSPHATE; TISSUE; SCAFFOLDS; HYDROXYAPATITE; APATITE; FIBERS; BIOMINERALIZATION; TRANSFORMATION; FABRICATION; MEMBRANES;

D O I：

10.1007/s10856-013-5133-9

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Mineralized poly(epsilon-caprolactone)/gelatin core-shell nanofibers were prepared via co-axial electrospinning and subsequent incubation in biomimetic simulated body fluid containing ten times the calcium and phosphate ion concentrations found in human blood plasma. The deposition of calcium phosphate on the nanofiber surfaces was investigated through scanning electronic microscopy and X-ray diffraction. Energy dispersive spectroscopy results indicated that calcium-deficient hydroxyapatite had grown on the fibers. Fourier transform infrared spectroscopy analysis suggested the presence of hydroxyl-carbonate-apatite. The results of a viability assay (MTT) and alkaline phosphatase activity analysis suggested that these mineralized matrices promote osteogenic differentiation of human adipose-derived stem cells (hASCs) when cultured in an osteogenic medium and have the potential to be used as a scaffold in bone tissue engineering. hASCs cultured in the presence of nanofibers in endothelial differentiation medium showed lower rates of proliferation than cells cultured without the nanofibers. However, endothelial cell markers were detected in cells cultured in the presence of nanofibers in endothelial differentiation medium.

引用

页码：1137 / 1148

页数：12

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