Association between gene and miRNA expression profiles and stereotyped subset #4 B-cell receptor in chronic lymphocytic leukemia

被引：21

作者：

Maura, Francesco ^{[1
,2
]}

Cutrona, Giovanna ^{[3
]}

Mosca, Laura ^{[1
,2
]}

Matis, Serena ^{[4
]}

Lionetti, Marta ^{[1
,2
]}

Fabris, Sonia ^{[1
,2
]}

Agnelli, Luca ^{[1
,2
]}

Colombo, Monica ^{[4
]}

Massucco, Carlotta ^{[4
]}

Ferracin, Manuela ^{[5
,6
]}

Zagatti, Barbara ^{[5
,6
]}

Reverberi, Daniele ^{[7
]}

Gentile, Massimo ^{[8
]}

Recchia, Anna Grazia ^{[8
]}

Bossio, Sabrina ^{[8
]}

Rossi, Davide ^{[9
]}

Gaidano, Gianluca ^{[9
]}

Molica, Stefano ^{[10
]}

Cortelezzi, Agostino ^{[1
,2
]}

Di Raimondo, Francesco ^{[11
,12
]}

Negrini, Massimo ^{[5
,6
]}

Tassone, Pierfrancesco ^{[13
]}

Morabito, Fortunato ^{[8
]}

Ferrarini, Manlio ^{[4
]}

Neri, Antonino ^{[1
,2
]}

机构：

[1] Univ Milan, Dept Clin Sci & Community Hlth, I-20122 Milan, Italy

[2] Fdn IRCCS Ca Granda, Osped Maggiore Policlin, Hematol CTMO 1, Milan, Italy

[3] IRCCS S Martino IST, SS Mol Diagnost, Genoa, Italy

[4] IRCCS S Martino IST, Sci Direct, Genoa, Italy

[5] Univ Ferrara, Dept Morphol Surg & Expt Med, I-44100 Ferrara, Italy

[6] Univ Ferrara, Lab Technol Adv Therapies LTTA, I-44100 Ferrara, Italy

[7] IRCCS S Martino IST, Dept Pathol, Genoa, Italy

[8] Azienda Osped Cosenza, UOC Ematol, Cosenza, Italy

[9] Amedeo Avogadro Univ Eastern Piedmont, Dept Translat Med, Div Hematol, Novara, Italy

[10] Pugliese Ciaccio Hosp, Dept Hematol & Oncol, Catanzaro, Italy

[11] Catania Univ, Catania, Italy

[12] Ferrarotto Hosp, Dept Biomed Sci, Div Hematol, Catania, Italy

[13] Magna Graecia Univ Catanzaro, Dept Expt & Clin Med, Catanzaro, Italy

来源：

LEUKEMIA & LYMPHOMA | 2015年 / 56卷 / 11期

关键词：

B-cell receptor; BCL2; chronic lymphocytic leukemia; gene expression profiling; microRNA; stereotyped VH CDR; CLINICAL-FEATURES; PROGRESSION; MUTATIONS; CANCER; IMMUNOGLOBULINS; MULTICENTER; GUIDELINES; DIAGNOSIS; APOPTOSIS; PATTERNS;

D O I：

10.3109/10428194.2015.1028051

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In this study we investigated specific biological and clinical features associated with chronic lymphocytic leukemia (CLL) patients carrying stereotyped BCR subset #4 (IGHV4-34) among a prospective cohort of 462 CLL/MBL patients in early stage (Binet A). All subset #4 patients (n = 16) were characterized by the IGHV mutated gene configuration, and absence of unfavorable cytogenetic lesions, NOTCH1 or SF3B1 mutations. Gene and miRNA expression profiling evidenced that the leukemic cells of subset #4 cases showed significant downregulation of WDFY4, MF2A and upregulation of PDGFA, FGFR1 and TFEC gene transcripts, as well as the upregulation of miR-497 and miR-29c. The transfection of miR-497 mimic in primary leukemic CLL cells induced a downregulation of BCL2, a known validated target of this miRNA. Our data identify biological characteristics associated with subset #4 patients, providing further evidence for the putative role of BCR in shaping the features of the tumor cells in CLL.

引用

页码：3150 / 3158

页数：9

共 45 条

[41] Immune Thrombocytopenia in Patients with Chronic Lymphocytic Leukemia Is Associated with Stereotyped B-cell Receptors [J].

Visco, Carlo ;

Maura, Francesco ;

Tuana, Giacomo ;

Agnelli, Luca ;

Lionetti, Marta ;

Fabris, Sonia ;

Novella, Elisabetta ;

Giaretta, Ilaria ;

Reda, Gianluigi ;

Barcellini, Wilma ;

Baldini, Luca ;

Neri, Antonino ;

Rodeghiero, Francesco ;

Cortelezzi, Agostino .

CLINICAL CANCER RESEARCH, 2012, 18 (07) :1870-1878

[42] miR-497 and miR-302b Regulate Ethanol-induced Neuronal Cell Death through BCL2 Protein and Cyclin D2 [J].

Yadav, Sanjay ;

Pandey, Ankita ;

Shukla, Aruna ;

Talwelkar, Sarang S. ;

Kumar, Ashutosh ;

Pant, Aditya B. ;

Parmar, Devendra .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (43) :37347-37357

[43] An intronic variant associated with systemic lupus erythematosus changes the binding affinity of Yinyang1 to downregulate WDFY4 [J].

Zhao, H. ;

Yang, W. ;

Qiu, R. ;

Li, J. ;

Xin, Q. ;

Wang, X. ;

Feng, Y. ;

Shan, S. ;

Liu, Y. ;

Gong, Y. ;

Liu, Q. .

GENES AND IMMUNITY, 2012, 13 (07) :536-542

[44] miR-497 modulates multidrug resistance of human cancer cell lines by targeting BCL2 [J].

Zhu, Wei ;

Zhu, DanXia ;

Lu, Shiqiang ;

Wang, Tongshan ;

Wang, Jian ;

Jiang, Binghua ;

Shu, Yongqian ;

Liu, Ping .

MEDICAL ONCOLOGY, 2012, 29 (01) :384-391

[45] Apoptosis or plasma cell differentiation of CD38-positive B-chronic lymphocytic leukemia cells induced by cross-linking of surface IgM or IgD [J].

Zupo, S ;

Massara, R ;

Dono, M ;

Rossi, E ;

Malavasi, F ;

Cosulich, ME ;

Ferrarini, M .

BLOOD, 2000, 95 (04) :1199-1206

← 1 2 3 4 5 →