Association between gene and miRNA expression profiles and stereotyped subset #4 B-cell receptor in chronic lymphocytic leukemia

被引：21

作者：

Maura, Francesco ^{[1
,2
]}

Cutrona, Giovanna ^{[3
]}

Mosca, Laura ^{[1
,2
]}

Matis, Serena ^{[4
]}

Lionetti, Marta ^{[1
,2
]}

Fabris, Sonia ^{[1
,2
]}

Agnelli, Luca ^{[1
,2
]}

Colombo, Monica ^{[4
]}

Massucco, Carlotta ^{[4
]}

Ferracin, Manuela ^{[5
,6
]}

Zagatti, Barbara ^{[5
,6
]}

Reverberi, Daniele ^{[7
]}

Gentile, Massimo ^{[8
]}

Recchia, Anna Grazia ^{[8
]}

Bossio, Sabrina ^{[8
]}

Rossi, Davide ^{[9
]}

Gaidano, Gianluca ^{[9
]}

Molica, Stefano ^{[10
]}

Cortelezzi, Agostino ^{[1
,2
]}

Di Raimondo, Francesco ^{[11
,12
]}

Negrini, Massimo ^{[5
,6
]}

Tassone, Pierfrancesco ^{[13
]}

Morabito, Fortunato ^{[8
]}

Ferrarini, Manlio ^{[4
]}

Neri, Antonino ^{[1
,2
]}

机构：

[1] Univ Milan, Dept Clin Sci & Community Hlth, I-20122 Milan, Italy

[2] Fdn IRCCS Ca Granda, Osped Maggiore Policlin, Hematol CTMO 1, Milan, Italy

[3] IRCCS S Martino IST, SS Mol Diagnost, Genoa, Italy

[4] IRCCS S Martino IST, Sci Direct, Genoa, Italy

[5] Univ Ferrara, Dept Morphol Surg & Expt Med, I-44100 Ferrara, Italy

[6] Univ Ferrara, Lab Technol Adv Therapies LTTA, I-44100 Ferrara, Italy

[7] IRCCS S Martino IST, Dept Pathol, Genoa, Italy

[8] Azienda Osped Cosenza, UOC Ematol, Cosenza, Italy

[9] Amedeo Avogadro Univ Eastern Piedmont, Dept Translat Med, Div Hematol, Novara, Italy

[10] Pugliese Ciaccio Hosp, Dept Hematol & Oncol, Catanzaro, Italy

[11] Catania Univ, Catania, Italy

[12] Ferrarotto Hosp, Dept Biomed Sci, Div Hematol, Catania, Italy

[13] Magna Graecia Univ Catanzaro, Dept Expt & Clin Med, Catanzaro, Italy

来源：

LEUKEMIA & LYMPHOMA | 2015年 / 56卷 / 11期

关键词：

B-cell receptor; BCL2; chronic lymphocytic leukemia; gene expression profiling; microRNA; stereotyped VH CDR; CLINICAL-FEATURES; PROGRESSION; MUTATIONS; CANCER; IMMUNOGLOBULINS; MULTICENTER; GUIDELINES; DIAGNOSIS; APOPTOSIS; PATTERNS;

D O I：

10.3109/10428194.2015.1028051

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In this study we investigated specific biological and clinical features associated with chronic lymphocytic leukemia (CLL) patients carrying stereotyped BCR subset #4 (IGHV4-34) among a prospective cohort of 462 CLL/MBL patients in early stage (Binet A). All subset #4 patients (n = 16) were characterized by the IGHV mutated gene configuration, and absence of unfavorable cytogenetic lesions, NOTCH1 or SF3B1 mutations. Gene and miRNA expression profiling evidenced that the leukemic cells of subset #4 cases showed significant downregulation of WDFY4, MF2A and upregulation of PDGFA, FGFR1 and TFEC gene transcripts, as well as the upregulation of miR-497 and miR-29c. The transfection of miR-497 mimic in primary leukemic CLL cells induced a downregulation of BCL2, a known validated target of this miRNA. Our data identify biological characteristics associated with subset #4 patients, providing further evidence for the putative role of BCR in shaping the features of the tumor cells in CLL.

引用

页码：3150 / 3158

页数：9

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