Amperometric hydrogen peroxide biosensor based on the immobilization of horseradish peroxidase by carbon-coated iron nanoparticles in combination with chitosan and cross-linking of glutaraldehyde

被引：36

作者：

Lai, Guo-Song ^{[1
]}

Zhang, Hai-Li ^{[1
]}

Han, De-Yan ^{[1
]}

机构：

[1] Hubei Normal Univ, Hubei Key Lab Bioanalyt Tech, Dept Chem & Environm Engn, Huangshi 435002, Peoples R China

来源：

MICROCHIMICA ACTA | 2009年 / 165卷 / 1-2期

关键词：

Horseradish peroxidase; Carbon-coated iron nanoparticles; Hydrogen peroxide; Biosensor; Polythionine; DIRECT ELECTROCHEMISTRY; MODIFIED ELECTRODE; GOLD NANOPARTICLES; COMPOSITE FILM; VOLTAMMETRIC DETERMINATION; FE3O4; NANOPARTICLES; NANOTUBES; THIONINE; HEMOGLOBIN; MICROSPHERES;

D O I：

10.1007/s00604-008-0114-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Magnetic carbon-coated iron nanoparticles were used to immobilize horseradish peroxidase on the surface of a polythionine modified glassy carbon electrode in combination with chitosan and cross-linking of glutaraldehyde. The electrochemical character of this enzyme electrode and its electrocatalytic reduction to H2O2 were studied by cyclic voltammetry. The effects of the common experimental variables were investigated. Under the optimum conditions, this method could be successfully used for the amperometric determination of H2O2 in a wide concentration range of 9.6 mu M to 3.16 mM with a detection limit of 3.6 mu M (S/N=3). Besides, the biosensor also exhibited good selectivity, stability and reproducibility.

引用

页码：159 / 165

页数：7

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