A comparative rapid and sensitive method to screen L-asparaginase producing fungi

被引：19

作者：

Dhale, Mohan A. ^{[1
]}

Mohan-Kumari, H. Puttananjaiah ^{[2
]}

机构：

[1] CSIR, Cent Food Technol Res Inst, Dept Food Microbiol, Mysore 570020, Karnataka, India

[2] CSIR, Cent Food Technol Res Inst, Dept Grain Sci & Technol, Mysore 570020, Karnataka, India

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2014年 / 102卷

关键词：

L-asparaginase; Phenol red; Bromothymol blue; Methyl red; PLATE ASSAY; MICROORGANISMS;

D O I：

10.1016/j.mimet.2014.04.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fungi are well known to produce various industrial enzymes and secondary metabolites with different colours. Fungi producing L-asparaginase enzyme are conventionally screened on medium containing phenol red (PR). The contrast between enzyme-hydrolysed zone and unhydrolysed L-asparagine is not very evident and distinct in medium containing PR and bromothymol blue (BB) due to coloured secondary metabolite production. Thus, PR and BB limit and affect the detection and screening method. In the present investigation, an improved method for screening is reported by comparing with PR and BB, wherein methyl red (MR) is incorporated as pH indicator. The enzyme activity was distinctly observed (red and light-yellow) in MR incorporated medium compared to PR and BB. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：66 / 68

页数：3

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