Arabidopsis HSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses

被引:64
作者
Bao, Fei [1 ]
Huang, Xiaozhen [1 ]
Zhu, Chipan [2 ]
Zhang, Xiaoyan [1 ]
Li, Xin [2 ]
Yang, Shuhua [1 ]
机构
[1] China Agr Univ, State Key Lab Plant Physiol & Biochem, Coll Biol Sci, Natl Plant Gene Res Ctr, Beijing 100193, Peoples R China
[2] Univ British Columbia, Michael Smith Labs, Vancouver, BC V5Z 1M9, Canada
基金
中国国家自然科学基金;
关键词
Arabidopsis; cell death; HSP90; proteins; low temperature; RPP4; PLANT INNATE IMMUNITY; DISEASE RESISTANCE GENES; OF-FUNCTION MUTATION; SALICYLIC-ACID; FUNCTIONAL-ANALYSIS; TIR DOMAIN; NUCLEAR ACCUMULATION; PATHOGEN RESISTANCE; SIGNALING PROTEINS; FUNGAL PATHOGEN;
D O I
10.1111/nph.12760
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
<list list-type="bulleted" id="nph12760-list-0001"> Plant defense responses are regulated by temperature. In Arabidopsis, the chilling-sensitive mutant chs2-1 (rpp4-1d) contains a gain-of-function mutation in the TIR-NB-LRR (Toll and interleukin 1 receptor-nucleotide binding-leucine-rich repeat) gene, RPP4 (RECOGNITION OF PERONOSPORA PARASITICA 4), which leads to constitutive activation of the defense response at low temperatures. Here, we identified and characterized two suppressors of rpp4-1d from a genetic screen, hsp90.2 and hsp90.3, which carry point mutations in the cytosolic heat shock proteins HSP90.2 and HSP90.3, respectively. The hsp90 mutants suppressed the chilling sensitivity of rpp4-1d, including seedling lethality, activation of the defense responses and cell death under chilling stress. The hsp90 mutants exhibited compromised RPM1 (RESISTANCE TO PSEUDOMONAS MACULICOLA 1)-, RPS4 (RESISTANCE TO P. SYRINGAE 4)- and RPP4-mediated pathogen resistance. The wild-type RPP4 and the mutated form rpp4 could interact with HSP90 to form a protein complex. Furthermore, RPP4 and rpp4 proteins accumulated in the cytoplasm and nucleus at normal temperatures, whereas the nuclear accumulation of the mutated rpp4 was decreased at low temperatures. Genetic analysis of the intragenic suppressors of rpp4-1d revealed the important functions of the NB-ARC and LRR domains of RPP4 in temperature-dependent defense signaling. In addition, the rpp4-1d-induced chilling sensitivity was largely independent of the WRKY70 or MOS (modifier of snc1) genes. [Correction added after online publication 11 March 2013: the expansions of TIR-NB-LRR and RPS4 were amended] This study reveals that Arabidopsis HSP90 regulates RPP4-mediated temperature-dependent cell death and defense responses.
引用
收藏
页码:1320 / 1334
页数:15
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