Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production

被引：36

作者：

Browning, Douglas F. ^{[1
]}

Godfrey, Rita E. ^{[1
]}

Richards, Kirsty L. ^{[2
]}

Robinson, Colin ^{[2
]}

Busby, Stephen J. W. ^{[1
]}

机构：

[1] Univ Birmingham, Sch Biosci, Inst Microbiol & Infect, Birmingham B15 2TT, W Midlands, England

[2] Univ Kent, Sch Biosci, Ingram Bldg, Canterbury CT2 7NJ, Kent, England

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2019年 / 47卷

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会; “创新英国”项目;

关键词：

HIGH-LEVEL EXPRESSION; HUMAN GROWTH-HORMONE; TRANSCRIPTION INITIATION; RNA-POLYMERASE; TAC PROMOTER; REPRESSOR; VECTORS; SYSTEM; OPERATORS; SEQUENCES;

D O I：

10.1042/BST20190059

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here, we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give a controlled expression that is suitable for diverse biotechnology applications.

引用

页码：755 / 763

页数：9

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