Influence of polyelectrolyte on dynamic surface properties of BSA solutions

被引：10

作者：

Milyaeva, Olga Yu. ^{[1
]}

Noskov, Boris A. ^{[1
]}

Lin, Shi-Yow ^{[2
]}

Loglio, Giuseppe ^{[3
]}

Miller, Reinhard ^{[4
]}

机构：

[1] St Petersburg State Univ, Dept Colloid Chem, St Petersburg 198504, Russia

[2] Natl Taiwan Univ Sci & Technol, Dept Chem Engn, Taipei 106, Taiwan

[3] Univ Firenze, Dipartimento Chim Organ, I-50019 Florence, Italy

[4] MPI Kolloid & Grenzflachenforsch, D-14424 Golm, Germany

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 2014年 / 442卷

关键词：

Protein polyelectrolyte interactions; Adsorption kinetics; Surface dilational elasticity; Dynamic surface tension; Protein unfolding; BOVINE SERUM-ALBUMIN; LIGHT-SCATTERING; AIR/WATER INTERFACE; ADSORPTION LAYER; PROTEIN; BINDING; COMPLEXATION; ELASTICITY; RHEOLOGY; GELS;

D O I：

10.1016/j.colsurfa.2013.05.023

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The dynamic surface elasticity of mixed solutions of bovine serum albumin (BSA) and poly(diallyldimethylammonium chloride) (PDADMAC) was measured as a function of the surface age, polyelectrolyte concentration and solution pH. The addition of polyelectrolyte does not influence the dynamic surface properties of protein solution at pH below the isoelectric point of BSA. At the same time, one can observe significant changes of the kinetic dependencies of the surface pressure and surface elasticity under the influence of polyelectrolyte at higher pH indicating the interaction between the components. The acceleration of the changes of the surface properties can be connected with the decrease of the electric adsorption barrier due to the decrease of the total charge of the protein/polyelectrolyte complex. The local maximum of the kinetic dependencies of the dynamic surface elasticity at pH higher than the isoelectric point indicates partial destruction of the protein tertiary structure at high surface pressures. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：63 / 68

页数：6

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