Fentanyl inhibits glucose-stimulated insulin release from β-cells in rat pancreatic islets

被引:6
作者
Qian, Tao-Lai [1 ]
Wang, Xin-Hua [1 ]
Liu, Sheng [2 ]
Ma, Liang [3 ]
Lu, Ying [4 ]
机构
[1] Tongji Univ, Dept Pain Management, Shanghai E Hosp, Shanghai 2000120, Peoples R China
[2] Tongji Univ, Dept Anesthesia, Shanghai E Hosp, Shanghai 200120, Peoples R China
[3] Tongji Univ, Cell Therapy Ctr, Shanghai E Hosp, Shanghai 200120, Peoples R China
[4] Tongji Univ, Cent Lab, Shanghai E Hosp, Shanghai 200120, Peoples R China
关键词
Fentanyl; Inhibition; Insulin release; Islets; TRANSDERMAL FENTANYL; OPIOID-PEPTIDES; CANCER PAIN; MTT ASSAY; IN-VITRO; SECRETION; MODULATION; ENDORPHIN; ACTIVATION; EXPRESSION;
D O I
10.3748/wjg.15.4163
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
AIM: To explore the effects of fentanyl on insulin release from freshly isolated rat pancreatic islets in static culture. METHODS: Islets were isolated from the pancreas of mature Sprague Dawley rats by common bile duct intraductal collagenase V digestion and were purified by discontinuous Ficoll density gradient centrifugation. The islets were divided into four groups according to the fentanyl concentration: control group (0 ng/mL), group I (0.3 ng/mL), group II (3.0 ng/mL), and group M (30 ng/mL). In each group, the islets were co-cultured for 48 h with drugs under static conditions with fentanyl alone, fentanyl + 0.1 mu g/mL naloxone or fentanyl + 1.0 mu g/mL naloxone. Cell viability was assessed by the MTT assay. Insulin release in response to low and high concentrations (2.8 mmol/L and 16.7 mmol/L, respectively) of glucose was investigated and electron microscopy morphological assessment was performed. RESULTS: Low- and high-glucose-stimulated insulin release in the control group was significantly higher than in groups II and III (62.33 +/- 9.67 mu IU vs 47.75 +/- 8.47 mu IU, 39.67 +/- 6.18 mu IU and 125.5 +/- 22.04 mu IU vs 96.17 +/- 14.17 mu IU, 75.17 +/- 13.57 mu IU, respectively, P < 0.01) and was lowest in group III (P < 0.01). After adding 1 mu g/mL naloxone, insulin release in groups II and III was not different from the control group. Electron microscopy studies showed that the islets were damaged by 30 ng/mL fentanyl, CONCLUSION: Fentanyl inhibited glucose-stimulated insulin release from rat islets, which could be prevented by naloxone. Higher concentrations of fentanyl significantly damaged beta-cells of rat islets. (C) 2009 The WJG Press and Baishideng. All rights reserved.
引用
收藏
页码:4163 / 4169
页数:7
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