Prevalence and multilocus genotyping of potentially zoonotic Giardia duodenalis in pigs in Shanghai, China

被引：2
作者：
Liu, Hua [1 ,2 ,3 ,4 ,5 ]
Xu, Ning [1 ,2 ,3 ,4 ,5 ]
Yin, Jianhai [1 ,2 ,3 ,4 ,5 ]
Yuan, Zhongying [1 ,2 ,3 ,4 ,5 ]
Shen, Yujuan [1 ,2 ,3 ,4 ,5 ]
Cao, Jianping [1 ,2 ,3 ,4 ,5 ]
机构：
[1] Chinese Ctr Dis Control & Prevent, Natl Inst Parasit Dis, Shanghai 200025, Peoples R China
[2] Chinese Ctr Trop Dis Res, Shanghai 200025, Peoples R China
[3] WHO Collaborating Ctr Trop Dis, Shanghai 200025, Peoples R China
[4] Minist Sci & Technol, Natl Ctr Int Res Trop Dis, Shanghai 200025, Peoples R China
[5] MOH, Key Lab Parasite & Vector Biol, Shanghai 200025, Peoples R China
来源：
PARASITOLOGY | 2019年 / 146卷 / 09期
关键词：
Assemblage; bg; gdh; Giardia duodenalis; tpi; MOLECULAR CHARACTERIZATION; CRYPTOSPORIDIUM SPP; HUMAN INFECTION; IDENTIFICATION; TRANSMISSION; CATTLE; LOCUS; RISK; DOGS; ENTEROCYTOZOON;
D O I：
10.1017/S0031182019000349
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Giardia duodenalis is a common intestinal parasite in humans and other mammals, and it causes major public and veterinary health problems worldwide. China is a major pig-raising country, and studies on Giardia in pigs have important public health significance. The present study was conducted to investigate the prevalence of Giardia and assess its genetic characterization. A total of 93 samples were collected from two farms in Shanghai. The presence of Giardia was determined using PCR and sequence analysis of glutamate dehydrogenase, beta-giardin and triose phosphate isomerase genes. The average prevalence of G. duodenalis infection was 26.88% (25/93) in the pigs, with 28.13% (18/64) in farm 1 vs 24.14% (7/29) in farm 2. All the PCR-positive products were successfully sequenced, and assemblage E was more prevalent. Zoonotic assemblages A and B and canine-specific assemblage C were identified in farm 1, whereas, only assemblage E was detected in farm 2. Interestingly, two pig isolates showed 100% homology with human-derived isolates from Australia and China at the bg and tpi loci respectively. Pigs infected with Giardia infect humans by polluting the environment; whether pigs are a potential environmental source of the human pathogen in China requires more epidemiological data.
引用
收藏
页码：1199 / 1205
页数：7
相关论文
共 55 条
[31]   Genetic Analysis of the Gdh and Bg Genes of Animal-Derived Giardia duodenalis Isolates in Northeastern China and Evaluation of Zoonotic Transmission Potential [J].
Liu, Aiqin ;
Yang, Fengkun ;
Shen, Yujuan ;
Zhang, Weizhe ;
Wang, Rongjun ;
Zhao, Wei ;
Zhang, Longxian ;
Ling, Hong ;
Cao, Jianping .
PLOS ONE, 2014, 9 (04)
[32]   Prevalence and genetic characterization of Cryptosporidium, Enterocytozoon, Giardia and Cyclospora in diarrheal outpatients in china [J].
Liu, Hua ;
Shen, Yujuan ;
Yin, Jianhai ;
Yuan, Zhongying ;
Jiang, Yanyan ;
Xu, Yuxin ;
Pan, Wei ;
Hu, Yuan ;
Cao, Jianping .
BMC INFECTIOUS DISEASES, 2014, 14
[33]   Cryptosporidium and Giardia in different age groups of Danish cattle and pigs -: Occurrence and management associated risk factors [J].
Maddox-Hyttel, Charlotte ;
Langkjaer, Rikke B. ;
Enemark, Heidi L. ;
Vigre, Hakan .
VETERINARY PARASITOLOGY, 2006, 141 (1-2) :48-59
[34]   Occurrence and Diversity of Giardia duodenalis Assemblages in Livestock in the UK [J].
Minetti, C. ;
Taweenan, W. ;
Hogg, R. ;
Featherstone, C. ;
Randle, N. ;
Latham, S. M. ;
Wastling, J. M. .
TRANSBOUNDARY AND EMERGING DISEASES, 2014, 61 (06) :E60-E67
[35]  
Pand X, 2015, J TROPICAL MED, V15, P1438
[36]  
Pang XingLin Pang XingLin, 2015, Journal of Tropical Medicine (Guangzhou), V15, P1425
[37]   Cryptosporidium and Giardia in Danish organic pig farms: Seasonal and age-related variation in prevalence, infection intensity and species/genotypes [J].
Petersen, Heidi H. ;
Wang Jianmin ;
Katakam, Kiran K. ;
Mejer, Helena ;
Thamsborg, Stig M. ;
Dalsgaard, Anders ;
Olsen, Annette ;
Enemark, Heidi L. .
VETERINARY PARASITOLOGY, 2015, 214 (1-2) :29-39
[38]   First report on Giardia duodenalis assemblage F in Slovakian children living in poor environmental conditions [J].
Pipikova, Jana ;
Papajova, Ingrid ;
Majlathova, Viktoria ;
Soltys, Jindrich ;
Bystrianska, Julia ;
Schusterova, Ingrid ;
Vargova, Veronika .
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION, 2020, 53 (01) :148-156
[39]   Giardia and Cryptosporidium join the 'Neglected Diseases Initiative' [J].
Savioli, L. ;
Smith, H. ;
Thompson, A. .
TRENDS IN PARASITOLOGY, 2006, 22 (05) :203-208
[40]   Comparisons of mammalian Giardia duodenalis assemblages based on the β-giardin, glutamate dehydrogenase and triose phosphate isomerase genes [J].
Scorza, Andrea V. ;
Ballweber, Lora R. ;
Tangtrongsup, Sahatchai ;
Panuska, Carla ;
Lappin, Michael R. .
VETERINARY PARASITOLOGY, 2012, 189 (2-4) :182-188
123456