Prevalence and multilocus genotyping of potentially zoonotic Giardia duodenalis in pigs in Shanghai, China

被引：2
作者：
Liu, Hua [1 ,2 ,3 ,4 ,5 ]
Xu, Ning [1 ,2 ,3 ,4 ,5 ]
Yin, Jianhai [1 ,2 ,3 ,4 ,5 ]
Yuan, Zhongying [1 ,2 ,3 ,4 ,5 ]
Shen, Yujuan [1 ,2 ,3 ,4 ,5 ]
Cao, Jianping [1 ,2 ,3 ,4 ,5 ]
机构：
[1] Chinese Ctr Dis Control & Prevent, Natl Inst Parasit Dis, Shanghai 200025, Peoples R China
[2] Chinese Ctr Trop Dis Res, Shanghai 200025, Peoples R China
[3] WHO Collaborating Ctr Trop Dis, Shanghai 200025, Peoples R China
[4] Minist Sci & Technol, Natl Ctr Int Res Trop Dis, Shanghai 200025, Peoples R China
[5] MOH, Key Lab Parasite & Vector Biol, Shanghai 200025, Peoples R China
来源：
PARASITOLOGY | 2019年 / 146卷 / 09期
关键词：
Assemblage; bg; gdh; Giardia duodenalis; tpi; MOLECULAR CHARACTERIZATION; CRYPTOSPORIDIUM SPP; HUMAN INFECTION; IDENTIFICATION; TRANSMISSION; CATTLE; LOCUS; RISK; DOGS; ENTEROCYTOZOON;
D O I：
10.1017/S0031182019000349
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Giardia duodenalis is a common intestinal parasite in humans and other mammals, and it causes major public and veterinary health problems worldwide. China is a major pig-raising country, and studies on Giardia in pigs have important public health significance. The present study was conducted to investigate the prevalence of Giardia and assess its genetic characterization. A total of 93 samples were collected from two farms in Shanghai. The presence of Giardia was determined using PCR and sequence analysis of glutamate dehydrogenase, beta-giardin and triose phosphate isomerase genes. The average prevalence of G. duodenalis infection was 26.88% (25/93) in the pigs, with 28.13% (18/64) in farm 1 vs 24.14% (7/29) in farm 2. All the PCR-positive products were successfully sequenced, and assemblage E was more prevalent. Zoonotic assemblages A and B and canine-specific assemblage C were identified in farm 1, whereas, only assemblage E was detected in farm 2. Interestingly, two pig isolates showed 100% homology with human-derived isolates from Australia and China at the bg and tpi loci respectively. Pigs infected with Giardia infect humans by polluting the environment; whether pigs are a potential environmental source of the human pathogen in China requires more epidemiological data.
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页码：1199 / 1205
页数：7
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