Characterizing vascular parameters in hypoxic regions: A combined magnetic resonance and optical imaging study of a human prostate cancer model

被引:55
作者
Raman, Venu
Artemov, Dmitri
Pathak, Arvind P.
Winnard, Paul T., Jr.
McNutt, Stephen
Yudina, Anna
Bogdanov, Alexei, Jr.
Bhujwalla, Zaver M.
机构
[1] Johns Hopkins Univ, Sch Med, Russell H Morgan Dept Radiol & Radiol Sci, Johns Hopkins Univ In Vivo Cellular Mol Imaging C, Baltimore, MD 21205 USA
[2] Univ Massachusetts, Sch Med, Dept Radiol, Worcester, MA USA
关键词
D O I
10.1158/0008-5472.CAN-06-0886
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The integration of imaging technologies with the capabilities of genetic engineering has created novel opportunities for understanding and imaging cancer. Here, we have combined vascular magnetic resonance imaging (MRI) and optical imaging to understand the relationship between hypoxia and vascularization in a human prostate cancer model engineered to express enhanced green fluorescent protein (EGFP) under hypoxia. Characterization and validation of EGFP expression under hypoxic conditions was done in culture and in solid tumors in vivo. MRI measurements showed that vascular volume was significantly lower in fluorescing regions. These regions also frequently exhibited high permeability. These data were further supported by the detection of low vessel density in EGFP-positive regions, as determined by the distribution of intravascularly administered, fluorescence-labeled Lycopersicon esculentum lectin in frozen tumor sections. These observations are consistent with the possibility that regions of low vascular volumes are hypoxic, which induces increased expression of functionally active vascular endothelial growth factor, a potent vascular permeability factor.
引用
收藏
页码:9929 / 9936
页数:8
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