Sarco/endoplasmic reticulum Ca-2(+)-ATPase (SERCA2a) is important for cardiac physiological function and pathological progression. However, intravenous injection, a commonly applied approach for gene delivery in most studies investigating the expression of SERCA2a in cardiomyocytes, has not been particularly satisfactory. Therefore, in the present study, a modified method was used to transfect this gene into the heart. Specifically, a SERCA2a-knockdown lentivirus was directly injected into the myocardium of adult rats under ultrasound guidance, following which the effectiveness and feasibility of this proposed approach were evaluated. The results demonstrated that compared with traditional intravenous injection, the modified gene delivery method resulted in markedly higher transfection efficiency. In addition, the SERCA2a-knockdown rats exhibited higher rates of arrhythmia and weaker ventricular wall motions compared with those in the control rats, with these symptoms more evident in the rats that received a direct injection into the myocardium compared with those that were intravenously injected. These results suggest that ultrasound-guided injection into the myocardium is an efficient and safe method for gene delivery and for inducing the knockdown of SERCA2a protein expression in cardiomyocytes in their native environment.
机构:
Mt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USAMt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USA
Kho, Changwon
Lee, Ahyoung
论文数: 0引用数: 0
h-index: 0
机构:
Mt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USAMt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USA
Lee, Ahyoung
Jeong, Dongtak
论文数: 0引用数: 0
h-index: 0
机构:
Mt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USAMt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USA
Jeong, Dongtak
Oh, Jae Gyun
论文数: 0引用数: 0
h-index: 0
机构:
Gwangju Inst Sci & Technol, Coll Life Sci, Kwangju 500712, South KoreaMt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USA
Oh, Jae Gyun
Chaanine, Antoine H.
论文数: 0引用数: 0
h-index: 0
机构:
Mt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USAMt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USA
Chaanine, Antoine H.
Kizana, Eddy
论文数: 0引用数: 0
h-index: 0
机构:
Westmead Hosp, Westmead Millennium Inst, Sydney Med Sch, Westmead, NSW 2145, Australia
Westmead Hosp, Dept Cardiol, Westmead, NSW 2145, AustraliaMt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USA
Kizana, Eddy
Park, Woo Jin
论文数: 0引用数: 0
h-index: 0
机构:
Gwangju Inst Sci & Technol, Coll Life Sci, Kwangju 500712, South KoreaMt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USA
Park, Woo Jin
Hajjar, Roger J.
论文数: 0引用数: 0
h-index: 0
机构:
Mt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USAMt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USA
机构:
Gwathmey Inc, Cambridge, MA USAMt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USA
Gwathmey, Judith K.
Yerevanian, Alexan I.
论文数: 0引用数: 0
h-index: 0
机构:
Mt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USA
Univ Calif Los Angeles, Los Angeles, CA 90095 USAMt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USA
Yerevanian, Alexan I.
Hajjar, Roger J.
论文数: 0引用数: 0
h-index: 0
机构:
Mt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USAMt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY 10029 USA
机构:
Xuzhou Med Univ, Inst Cardiovasc Dis Res, 84 West Huaihai Rd, Xuzhou 221002, Jiangsu, Peoples R China
Jiangnan Univ, Affiliated Hosp, Dept Cardiol, Wuxi, Peoples R ChinaXuzhou Med Univ, Inst Cardiovasc Dis Res, 84 West Huaihai Rd, Xuzhou 221002, Jiangsu, Peoples R China
Zhai, Yuting
Chen, Junhong
论文数: 0引用数: 0
h-index: 0
机构:
Xuzhou Med Univ, Affiliated Hosp, Dept Cardiol, 1000 Hefeng Rd, Wuxi 214062, Jiangsu, Peoples R ChinaXuzhou Med Univ, Inst Cardiovasc Dis Res, 84 West Huaihai Rd, Xuzhou 221002, Jiangsu, Peoples R China
Chen, Junhong
Kan, Rongsheng
论文数: 0引用数: 0
h-index: 0
机构:
Xuzhou Med Univ, Inst Cardiovasc Dis Res, 84 West Huaihai Rd, Xuzhou 221002, Jiangsu, Peoples R ChinaXuzhou Med Univ, Inst Cardiovasc Dis Res, 84 West Huaihai Rd, Xuzhou 221002, Jiangsu, Peoples R China
Kan, Rongsheng
Xuan, Haochen
论文数: 0引用数: 0
h-index: 0
机构:
Xuzhou Med Univ, Affiliated Hosp, Dept Cardiol, 1000 Hefeng Rd, Wuxi 214062, Jiangsu, Peoples R ChinaXuzhou Med Univ, Inst Cardiovasc Dis Res, 84 West Huaihai Rd, Xuzhou 221002, Jiangsu, Peoples R China
Xuan, Haochen
Wang, Chaofan
论文数: 0引用数: 0
h-index: 0
机构:
Xuzhou Med Univ, Affiliated Hosp, Dept Cardiol, 1000 Hefeng Rd, Wuxi 214062, Jiangsu, Peoples R ChinaXuzhou Med Univ, Inst Cardiovasc Dis Res, 84 West Huaihai Rd, Xuzhou 221002, Jiangsu, Peoples R China
Wang, Chaofan
Li, Dongye
论文数: 0引用数: 0
h-index: 0
机构:
Xuzhou Med Univ, Inst Cardiovasc Dis Res, 84 West Huaihai Rd, Xuzhou 221002, Jiangsu, Peoples R ChinaXuzhou Med Univ, Inst Cardiovasc Dis Res, 84 West Huaihai Rd, Xuzhou 221002, Jiangsu, Peoples R China
Li, Dongye
Xu, Tongda
论文数: 0引用数: 0
h-index: 0
机构:
Xuzhou Med Univ, Affiliated Hosp, Dept Cardiol, 1000 Hefeng Rd, Wuxi 214062, Jiangsu, Peoples R ChinaXuzhou Med Univ, Inst Cardiovasc Dis Res, 84 West Huaihai Rd, Xuzhou 221002, Jiangsu, Peoples R China