A novel hydrogen peroxide biosensor based on hemoglobin-collagen-CNTs composite nanofibers

被引：28

作者：

Li, J. ^{[1
]}

Mei, H. ^{[1
]}

Zheng, W. ^{[2
]}

Pan, P. ^{[1
]}

Sun, X. J. ^{[1
]}

Li, F. ^{[1
]}

Guo, F. ^{[2
]}

Zhou, H. M. ^{[2
]}

Ma, J. Y. ^{[2
]}

Xu, X. X. ^{[2
]}

Zheng, Y. F. ^{[2
,3
]}

机构：

[1] Harbin Univ Sci & Technol, Key Lab Green Chem Engn & Technol, Coll Heilongjiang Prov, Coll Chem & Environm Engn, Harbin 150080, Peoples R China

[2] Harbin Engn Univ, Ctr Biomed Mat & Engn, Harbin 150001, Peoples R China

[3] Peking Univ, Coll Engn, Dept Mat Sci & Engn, Beijing 100871, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2014年 / 118卷

关键词：

Electrospinning; Hemoglobin-collagen-CNTs nanofibers; Electrochemistry; Biosensor; DIRECT ELECTRON-TRANSFER; DIRECT ELECTROCHEMISTRY; HORSERADISH-PEROXIDASE; CARBON; ELECTROCATALYSIS; FILM; NANOWIRE; OXIDASE; LIQUID; HB;

D O I：

10.1016/j.colsurfb.2014.03.035

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this paper, carbon nanotubes (CNTs) were successfully incorporated in the composite composed of hemoglobin (Hb) and collagen using co-electrospinning technology. The formed Hb-collagen-CNTs composite nanofibers possessed distinct advantage of three-dimensional porous structure, biocompatibility and excellent stability. The Hb immobilized in the electrospun nanofibers retained its natural structure and the heterogeneous electron transfer rate constant (k(s)) of the direct electron transfer between Hb and electrodes was 5.3 s(-1). In addition, the electrospun Hb-collagen-CNTs nanofibers modified electrodes showed good electrocatalytic properties toward H2O2 with a detection limit of 0.91 mu M (signal-to-noise ratio of 3) and the apparent Michaelis-Menten constant (K-m(app)) of 32.6 mu M. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：77 / 82

页数：6

共 46 条

[1] KINETICS OF THE REACTION OF COMPOUND-II OF HORSERADISH-PEROXIDASE WITH HYDROGEN-PEROXIDE TO FORM COMPOUND-III [J].