Development of polymorphic tetranucleotide microsatellites for pinyon jays (Gymnorhinus cyanocephalus)

被引：5

作者：

Busch, Joseph D. ^{[1
]}

Benford, Russell ^{[2
]}

Pearson, Talima ^{[2
]}

Palmer, Eldon ^{[2
]}

Balda, Russell P. ^{[2
]}

Keim, Paul ^{[2
]}

机构：

[1] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA

[2] No Arizona Univ, Dept Biol, Flagstaff, AZ 86011 USA

来源：

CONSERVATION GENETICS | 2009年 / 10卷 / 03期

基金：

美国国家科学基金会;

关键词：

Gymnorhinus cyanocephalus; Pinyon pine; Conservation; Hybridization enrichment; Tetranucleotide; LOCI;

D O I：

10.1007/s10592-008-9616-z

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

The pinyon jay (Gymnorhinus cyanocephalus) is a primary seed disperser of pinyon pines (Pinus edulis and P. monophylla). Both the pinyon jay and the pinyon pines are experiencing significant decline. While the pinyon jay is a species of management value and conservation concern, little is known about its fecundity, among-flock dispersal, and population differentiation. We initiated genetic studies in pinyon jays using a hybridization enrichment technique to isolate seven polymorphic microsatellite repeats (AAAG and GATA) from the pinyon jay genome. A locus from the Mexican jay (Aphelocoma ultramarina) that amplifies robustly in pinyon jays is also reported. These eight loci revealed moderate to high diversity in an Arizona population of pinyon jays (4-36 alleles and H (O) 0.42-0.90). As in other species, tetranucleotide repeats produced easily resolved amplification products.

引用

页码：689 / 691

页数：3

共 13 条

[1]

Balda Russell P., 2002, Birds of North America, V605, P1

[2] Modulation of non-templated nucleotide addition by taq DNA polymerase: Primer modifications that facilitate genotyping [J].

Brownstein, MJ ;

Carpten, JD ;

Smith, JR .

BIOTECHNIQUES, 1996, 20 (06) :1004-+

[3] Mortality gradients within and among dominant plant populations as barometers of ecosystem change during extreme drought [J].

Gitlin, Alicyn R. ;

Sthultz, Christopher M. ;

Bowker, Matthew A. ;

Stumpf, Stacy ;

Paxton, Kristina L. ;

Kennedy, Karla ;

Munoz, Axhel ;

Bailey, Joseph K. ;

Whitham, Thomas G. .

CONSERVATION BIOLOGY, 2006, 20 (05) :1477-1486

[4]

*IUCN, 2007, 2007 IUCN RED LIST T

[5]

Lewis P.O., 2001, Genetic Data Analysis: Computer program for the analysis of allelic data

[6] Isolation of tetranucleotide microsatellites from the Mexican jay Aphelocoma ultramarina [J].

Li, SH ;

Huang, YJ ;

Brown, JL .

MOLECULAR ECOLOGY, 1997, 6 (05) :499-501

[7] REPRODUCTIVE INTERDEPENDENCE OF PINON JAYS AND PINON PINES [J].

LIGON, JD .

ECOLOGICAL MONOGRAPHS, 1978, 48 (02) :111-126

[8]

Marzluff J.M., 1992, The pinyon jay: Behavioral ecology of a cooperative corvid

[9] GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research [J].

Peakall, R ;

Smouse, PE .

MOLECULAR ECOLOGY NOTES, 2006, 6 (01) :288-295

[10] Empirical analyses of plant-climate relationships for the western United States [J].

Rehfeldt, Gerald E. ;

Crookston, Nicholas L. ;

Warwell, Marcus V. ;

Evans, Jeffrey S. .

INTERNATIONAL JOURNAL OF PLANT SCIENCES, 2006, 167 (06) :1123-1150

← 1 2 →