Light sheet microscopy

被引：0
|
作者：
Weber, Michael [1 ]
Mickoleit, Michaela [1 ]
Huisken, Jan [1 ]
机构：
[1] Max Planck Inst Mol Cell Biol & Genet MPI CBG, Dresden, Germany
来源：
QUANTITATIVE IMAGING IN CELL BIOLOGY | 2014年 / 123卷
关键词：
EMBRYONIC-DEVELOPMENT; RESOLUTION; ULTRAMICROSCOPY; DEEP;
D O I：
10.1016/B978-042-420138-5.00011-2
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective plane illumination microscopy is presented as an alternative to confocal microscopy due to several superior features such as high-speed full-frame acquisition, minimal phototoxicity, and multiview sample rotation. Based on our experience over the last 10 years, we summarize the key concepts in light sheet microscopy, typical implementations, and successful applications. In particular, sample mounting for long time-lapse imaging and the resulting challenges in data processing are discussed in detail.
引用
收藏
页码：193 / 215
页数：23
相关论文
共 50 条
  • [31] Light Sheet Microscopy to Measure Protein Dynamics
    Rieckher, Matthias
    JOURNAL OF CELLULAR PHYSIOLOGY, 2017, 232 (01) : 27 - 35
  • [32] Axially swept light-sheet microscopy
    Nature Methods, 2015, 12 (8) : 706 - 706
  • [33] Single-objective light sheet microscopy
    Rita Strack
    Nature Methods, 2021, 18 : 28 - 28
  • [34] Confocal multiview light-sheet microscopy
    de Medeiros, Gustavo
    Norlin, Nils
    Gunther, Stefan
    Albert, Marvin
    Panavaite, Laura
    Fiuza, Ulla-Maj
    Peri, Francesca
    Hiiragi, Takashi
    Krzic, Uros
    Hufnagel, Lars
    NATURE COMMUNICATIONS, 2015, 6
  • [35] Adaptable, illumination patterning light sheet microscopy
    Rory M. Power
    Jan Huisken
    Scientific Reports, 8
  • [36] A Novel Fast Volumetric Light Sheet Microscopy
    Sancataldo, Giuseppe
    Bianchini, Paolo
    Saggau, Peter
    Ramoino, Paola
    Diaspro, Alberto
    Duocastella, Marti
    BIOPHYSICAL JOURNAL, 2016, 110 (03) : 648A - 648A
  • [37] Applications of Light-Sheet Microscopy in Microdevices
    Albert-Smet, Ignacio
    Marcos-Vidal, Asier
    Jose Vaquero, Juan
    Desco, Manuel
    Munoz-Barrutia, Arrate
    Ripoll, Jorge
    FRONTIERS IN NEUROANATOMY, 2019, 13
  • [38] Light sheet microscopy with acoustic sample confinement
    Zhengyi Yang
    Katy L. H. Cole
    Yongqiang Qiu
    Ildikó M. L. Somorjai
    Philip Wijesinghe
    Jonathan Nylk
    Sandy Cochran
    Gabriel C. Spalding
    David A. Lyons
    Kishan Dholakia
    Nature Communications, 10
  • [39] Light-sheet microscopy at high resolution
    Reto Fiolka
    Nature Biotechnology, 2021, 39 : 1345 - 1346
  • [40] Single-objective light sheet microscopy
    Strack, Rita
    NATURE METHODS, 2021, 18 (01) : 28 - 28
12345