Global DNA methylation status in laryngeal cancer

被引：5

作者：

Stembalska, Agnieszka ^{[1
]}

Leszczynski, Przemyslaw ^{[1
,2
]}

Gil, Justyna ^{[1
]}

Ramsey, David ^{[3
]}

Pitala, Grzegorz ^{[4
]}

Maciejczyk, Adam ^{[5
]}

Fraczek, Marcin ^{[6
]}

机构：

[1] Wroclaw Med Univ, Dept Genet, PL-50367 Wroclaw, Poland

[2] Wroclaw Med Univ, Dept Biol & Med Parasitol, PL-50367 Wroclaw, Poland

[3] Univ Limerick, Dept Math & Stat, Limerick, Ireland

[4] 4th Mil Hosp, Dept Clin Otolaryngol, Wroclaw, Poland

[5] Lower Silesian Oncol Ctr, Wroclaw, Poland

[6] Wroclaw Med Univ, Dept Otolaryngol, PL-50367 Wroclaw, Poland

来源：

HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK | 2014年 / 36卷 / 03期

关键词：

SQUAMOUS-CELL CARCINOMA; CYTOGENETIC ABNORMALITIES; HUMAN-PAPILLOMAVIRUS; ALCOHOL-CONSUMPTION; NORMAL-TISSUES; FOLATE STATUS; LIFE-STYLE; HYPOMETHYLATION; HEAD; HYPERMETHYLATION;

D O I：

10.1002/hed.23315

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

Background. The purpose of our study was to evaluate if DNA methylation level in leukocytes may be used as a surrogate marker of genome methylation status in laryngeal cancer tissues. Methods. We evaluated global DNA methylation using an ultra performance liquid chromatography (UPLC)-based method to assess the total content of 5-methylcytosine (5 mC). Results. The study was performed on DNA isolated from cancer tissues, adjacent normal tissues, and peripheral blood leukocytes in a group of 72 patients with laryngeal cancer. DNA hypomethylation was found in tumor tissue (56%) and normal tissue (49%). There was a significant correlation between the levels of 5 mC in these 2 types of tissue. There was no significant DNA hypomethylation in blood. A negative correlation between tumor grade and blood levels of 5 mC was found. Conclusion. The level of leukocyte DNA methylation measured using total 5 mC content cannot be used as a surrogate marker for genome methylation status in laryngeal cancer tissues. © 2013 Wiley Periodicals, Inc.

引用

页码：419 / 424

页数：6

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