Global DNA methylation status in laryngeal cancer

被引：6
|
作者：
Stembalska, Agnieszka [1 ]
Leszczynski, Przemyslaw [1 ,2 ]
Gil, Justyna [1 ]
Ramsey, David [3 ]
Pitala, Grzegorz [4 ]
Maciejczyk, Adam [5 ]
Fraczek, Marcin [6 ]
机构：
[1] Wroclaw Med Univ, Dept Genet, PL-50367 Wroclaw, Poland
[2] Wroclaw Med Univ, Dept Biol & Med Parasitol, PL-50367 Wroclaw, Poland
[3] Univ Limerick, Dept Math & Stat, Limerick, Ireland
[4] 4th Mil Hosp, Dept Clin Otolaryngol, Wroclaw, Poland
[5] Lower Silesian Oncol Ctr, Wroclaw, Poland
[6] Wroclaw Med Univ, Dept Otolaryngol, PL-50367 Wroclaw, Poland
来源：
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK | 2014年 / 36卷 / 03期
关键词：
SQUAMOUS-CELL CARCINOMA; CYTOGENETIC ABNORMALITIES; HUMAN-PAPILLOMAVIRUS; ALCOHOL-CONSUMPTION; NORMAL-TISSUES; FOLATE STATUS; LIFE-STYLE; HYPOMETHYLATION; HEAD; HYPERMETHYLATION;
D O I：
10.1002/hed.23315
中图分类号：
R76 [耳鼻咽喉科学];
学科分类号：
100213 ;
摘要：
Background. The purpose of our study was to evaluate if DNA methylation level in leukocytes may be used as a surrogate marker of genome methylation status in laryngeal cancer tissues. Methods. We evaluated global DNA methylation using an ultra performance liquid chromatography (UPLC)-based method to assess the total content of 5-methylcytosine (5 mC). Results. The study was performed on DNA isolated from cancer tissues, adjacent normal tissues, and peripheral blood leukocytes in a group of 72 patients with laryngeal cancer. DNA hypomethylation was found in tumor tissue (56%) and normal tissue (49%). There was a significant correlation between the levels of 5 mC in these 2 types of tissue. There was no significant DNA hypomethylation in blood. A negative correlation between tumor grade and blood levels of 5 mC was found. Conclusion. The level of leukocyte DNA methylation measured using total 5 mC content cannot be used as a surrogate marker for genome methylation status in laryngeal cancer tissues. © 2013 Wiley Periodicals, Inc.
引用
收藏
页码：419 / 424
页数：6
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