Global DNA methylation status in laryngeal cancer

被引：5
|
作者：
Stembalska, Agnieszka [1 ]
Leszczynski, Przemyslaw [1 ,2 ]
Gil, Justyna [1 ]
Ramsey, David [3 ]
Pitala, Grzegorz [4 ]
Maciejczyk, Adam [5 ]
Fraczek, Marcin [6 ]
机构：
[1] Wroclaw Med Univ, Dept Genet, PL-50367 Wroclaw, Poland
[2] Wroclaw Med Univ, Dept Biol & Med Parasitol, PL-50367 Wroclaw, Poland
[3] Univ Limerick, Dept Math & Stat, Limerick, Ireland
[4] 4th Mil Hosp, Dept Clin Otolaryngol, Wroclaw, Poland
[5] Lower Silesian Oncol Ctr, Wroclaw, Poland
[6] Wroclaw Med Univ, Dept Otolaryngol, PL-50367 Wroclaw, Poland
来源：
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK | 2014年 / 36卷 / 03期
关键词：
SQUAMOUS-CELL CARCINOMA; CYTOGENETIC ABNORMALITIES; HUMAN-PAPILLOMAVIRUS; ALCOHOL-CONSUMPTION; NORMAL-TISSUES; FOLATE STATUS; LIFE-STYLE; HYPOMETHYLATION; HEAD; HYPERMETHYLATION;
D O I：
10.1002/hed.23315
中图分类号：
R76 [耳鼻咽喉科学];
学科分类号：
100213 ;
摘要：
Background. The purpose of our study was to evaluate if DNA methylation level in leukocytes may be used as a surrogate marker of genome methylation status in laryngeal cancer tissues. Methods. We evaluated global DNA methylation using an ultra performance liquid chromatography (UPLC)-based method to assess the total content of 5-methylcytosine (5 mC). Results. The study was performed on DNA isolated from cancer tissues, adjacent normal tissues, and peripheral blood leukocytes in a group of 72 patients with laryngeal cancer. DNA hypomethylation was found in tumor tissue (56%) and normal tissue (49%). There was a significant correlation between the levels of 5 mC in these 2 types of tissue. There was no significant DNA hypomethylation in blood. A negative correlation between tumor grade and blood levels of 5 mC was found. Conclusion. The level of leukocyte DNA methylation measured using total 5 mC content cannot be used as a surrogate marker for genome methylation status in laryngeal cancer tissues. © 2013 Wiley Periodicals, Inc.
引用
收藏
页码：419 / 424
页数：6
相关论文
共 50 条
  • [1] Global DNA Methylation Status of Colorectal Cancer Cells Exposed to Photodynamic Therapy
    Abrahamse, H.
    Vorster, L.
    Houreld, N.
    PROCEEDINGS OF THE 9TH WORLD ASSOCIATION FOR LASER THERAPY CONGRESS (WALT), 2013, : 1 - 4
  • [2] Global DNA methylation status of colorectal cancer cells exposed to photodynamic therapy
    Vorster, L.
    Houreld, N.
    Abrahamse, H.
    PROCEEDINGS OF SAIP2012: THE 57TH ANNUAL CONFERENCE OF THE SOUTH AFRICAN INSTITUTE OF PHYSICS, 2012, : 311 - 314
  • [3] Global DNA promoter methylation in cancer
    Plass, Christoph
    TOXICOLOGY LETTERS, 2009, 189 : S16 - S16
  • [4] An altered global DNA methylation status in women with depression
    Reszka, Edyta
    Jablonska, Ewa
    Lesicka, Monika
    Wieczorek, Edyta
    Kapelski, Pawel
    Szczepankiewicz, Aleksandra
    Pawlak, Joanna
    Dmitrzak-Weglarz, Monika
    JOURNAL OF PSYCHIATRIC RESEARCH, 2021, 137 : 283 - 289
  • [5] Global placental DNA methylation status and gestational diabetes
    Reichetzeder, C.
    Putra, S. Dwi
    Pfab, T.
    Slowinski, T.
    Neuber, C.
    Kleuser, B.
    Hocher, B.
    DIABETOLOGIA, 2016, 59 : S47 - S47
  • [6] Regulation of cancer-specific microRNA by aberrant DNA methylation in laryngeal cancer
    Yabe, Haruna
    Saito, Koichiro
    Kamimoto, Takahiro
    Kimura, Miwako
    Ogawa, Kaoru
    Zama, Takeru
    CANCER RESEARCH, 2010, 70
  • [7] Regulation of cancer-specific microRNA by aberrant DNA methylation in laryngeal cancer
    Yabe, Haruna
    Saito, Koichiro
    Kamimoto, Takahiro
    Kimura, Miwako
    Ogawa, Kaoru
    Zama, Takeru
    CANCER RESEARCH, 2010, 70
  • [8] Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation
    Arturo Pinzon-Cortes, Jairo
    Perna-Chaux, Angelina
    Steven Rojas-Villamizar, Nicolas
    Diaz-Basabe, Angelica
    Carolina Polania-Villanueva, Diana
    Fernanda Jacome, Maria
    Olimpo Mendivil, Carlos
    Groot, Helena
    Lopez-Segura, Valeriano
    ENDOCRINE CONNECTIONS, 2017, 6 (08): : 708 - 725
  • [9] Global DNA Methylation and DNA Methyltransferase Status Among Cigarette Smokers in Saudi
    Alameer, Areej
    Sultan, Samar
    LIFE-BASEL, 2025, 15 (02):
  • [10] DNA METHYLATION AND HORMONE RECEPTOR STATUS IN BREAST CANCER
    Rauscher, G. H.
    Ahsan, H.
    Kibriya, M.
    Dai, Y.
    Wiley, E.
    Kajdacsy-Balla, A.
    Macias, V.
    Levenson, V.
    Tonetti, D.
    Ehrlich, M.
    AMERICAN JOURNAL OF EPIDEMIOLOGY, 2011, 173 : S254 - S254
12345