Differential patterns of NOTCH1-4 receptor expression are markers of glioma cell differentiation

被引:40
作者
Dell'Albani, Paola [1 ]
Rodolico, Margherita [1 ]
Pellitteri, Rosalia [1 ]
Tricarichi, Elisa [1 ,2 ]
Torrisi, Sebastiano Alfio [1 ,3 ]
D'Antoni, Simona [1 ]
Zappia, Mario [4 ]
Albanese, Vincenzo [1 ,4 ]
Caltabiano, Rosario [5 ]
Platania, Nunzio [4 ]
Aronica, Eleonora [6 ,7 ]
Catania, Maria Vincenza [1 ,8 ]
机构
[1] Inst Neurol Sci, Catania, Italy
[2] Univ Catania, Dept Chem Sci, Sect Biochem & Mol Biol, Catania, Italy
[3] Univ Catania, Dept Clin & Mol Biomed, Sect Pharmacol & Biochem, Catania, Italy
[4] Univ Catania, Dept Neurosci, Catania, Italy
[5] Univ Catania, Sect Anat Pathol, Dept GF Ingrassia, Catania, Italy
[6] Univ Amsterdam, Acad Med Ctr, Dept Neuro Pathol, NL-1105 AZ Amsterdam, Netherlands
[7] SEIN, Epilepsy Inst Netherlands Fdn, Heemstede, Netherlands
[8] IRCCS Oasi Maria SS, Troina, EN, Italy
关键词
cell differentiation; gene expression; gliomas; glioma cell cultures; intermediate filaments; Notch receptors; SURVIVAL; LIGANDS; PROTEIN; SYSTEM; GROWTH;
D O I
10.1093/neuonc/not168
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background. Notch signaling is deregulated in human gliomas and may play a role in their malignancy. However, the role of each Notch receptor in glioma cell differentiation and progression is not clear. We examined the expression pattern of Notch receptors and compared it with differentiation markers in glioma cell lines, primary human cultures, and biopsies of different grades. Furthermore, the effects of a g-secretase inhibitor (GSI) on cell survival were assessed. Methods. Notch receptors and markers of cellular differentiation were analyzed by reverse transcriptase PCR, Western blotting, immunohistochemistry, and immunocytochemistry. GSI sensitivity was assessed in both cell lines and primary cultures grown as monolayers or tumorspheres, by MTT assay. Results. In cell lines, Notch1 and Notch2/4 levels paralleled those of glial fibrillary acidic protein (GFAP) and vimentin, respectively. In human gliomas and primary cultures, Notch1 was moderate/strong in low-grade tumors but weak in glioblastoma multiforme (GBM). Conversely, Notch4 increased from astrocytoma grade II to GBM. Primary GBM cultures grown in serum (monolayer) showed moderate/high levels of CD133, nestin, vimentin, and Notch4 and very low levels of GFAP and Notch1, which were reduced in tumorspheres. This effect was drastic for Notch4. GSI reduced cell survival with stronger effect in serum, whilst human primary cultures showed different sensitivity. Conclusion. Data from cell lines and human gliomas suggest a correlation between expression of Notch receptors and cell differentiation. Namely, Notch1 and Notch4 are markers of differentiated and less differentiated glioma cells, respectively. We propose Notch receptors as markers of glioma grading and possible prognostic factors.
引用
收藏
页码:204 / 216
页数:13
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