Prevalence of G6PD deficiency in selected populations from two previously high malaria endemic areas of Sri Lanka

被引：12

作者：

Gunawardena, Sharmini ^{[1
]}

Kapilananda, G. M. G. ^{[1
]}

Samarakoon, Dilhani ^{[1
]}

Maddevithana, Sashika ^{[1
]}

Wijesundera, Sulochana ^{[2
]}

Goonaratne, Lallindra V. ^{[3
]}

Karunaweera, Nadira D. ^{[1
]}

机构：

[1] Univ Colombo, Dept Parasitol, Fac Med, Colombo, Sri Lanka

[2] Univ Colombo, Dept Biochem, Fac Med, Colombo, Sri Lanka

[3] Univ Colombo, Dept Pathol, Fac Med, Colombo, Sri Lanka

来源：

PLOS ONE | 2017年 / 12卷 / 02期

基金：

美国国家科学基金会;

关键词：

METHEMOGLOBIN REDUCTION TEST; GLUCOSE-6-PHOSPHATE-DEHYDROGENASE DEFICIENCY; X-CHROMOSOME; PRIMAQUINE; VARIANTS; GENE; DAPSONE; MUTATIONS; HEMOLYSIS; PROVINCE;

D O I：

10.1371/journal.pone.0171208

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Glucose-6-Phosphate Dehydrogenase (G6PD) enzyme deficiency is known to offer protection against malaria and an increased selection of mutant genes in malaria endemic regions is expected. However, anti-malarial drugs such as primaquine can cause haemolytic anaemia in persons with G6PD deficiency. We studied the extent of G6PD deficiency in selected persons attending Teaching Hospitals of Anuradhapura and Kurunegala, two previously high malaria endemic districts in Sri Lanka. A total of 2059 filter-paper blood spots collected between November 2013 and June 2014 were analysed for phenotypic G6PD deficiency using the modified WST-8/1-methoxy PMS method. Each assay was conducted with a set of controls and the colour development assessed visually as well as with a microplate reader at OD 450-630 nm. Overall, 142/1018 (13.95%) and 83/1041 (7.97%) were G6PD deficient in Anuradhapura and Kurunegala districts respectively. The G6PD prevalence was significantly greater in Anuradhapura when compared to Kurunegala (P<0.0001). Surprisingly, females were equally affected as males in each district: 35/313 (11.18%) males and 107/705 (15.18%) females were affected in Anuradhapura (P = 0.089); 25/313 (7.99%) males and 58/728 (7.97%) females were affected in Kurunegala (P = 0.991). Prevalence was greater among females in Anuradhapura than in Kurunegala (P<0.05), while no such difference was observed between the males (P>0.05). Severe deficiency (<10% normal) was seen among 28/1018 (2.75%) in Anuradhapura (7 males; 21 females) and 17/1041 (1.63%) in Kurunegala (7 males; 10 females). Enzyme activity between 10-30% was observed among 114/1018 (11.20%; 28 males; 86 females) in Anuradhapura while it was 66/1041 (6.34%; 18 males; 48 females) in Kurunegala. Screening and educational programmes for G6PD deficiency are warranted in these high risk areas irrespective of gender for the prevention of disease states related to this condition.

引用

页数：13

共 52 条

[31] Positively Selected G6PD-Mahidol Mutation Reduces Plasmodium vivax Density in Southeast Asians [J].

Louicharoen, Chalisa ;

Patin, Etienne ;

Paul, Richard ;

Nuchprayoon, Issarang ;

Witoonpanich, Bhee ;

Peerapittayamongkol, Chayanon ;

Casademont, Isabelle ;

Sura, Thanyachai ;

Laird, Nan M. ;

Singhasivanon, Pratap ;

Quintana-Murci, Lluis ;

Sakuntabhai, Anavaj .

SCIENCE, 2009, 326 (5959) :1546-1549

[32]

Luzzatto L, ONLINE METABOLIC MOL

[33] GENE ACTION IN X-CHROMOSOME OF MOUSE (MUS MUSCULUS L) [J].

LYON, MF .

NATURE, 1961, 190 (477) :372-&

[34] STRUCTURAL-ANALYSIS OF THE X-LINKED GENE ENCODING HUMAN GLUCOSE-6-PHOSPHATE-DEHYDROGENASE [J].

MARTINI, G ;

TONIOLO, D ;

VULLIAMY, T ;

LUZZATTO, L ;

DONO, R ;

VIGLIETTO, G ;

PAONESSA, G ;

DURSO, M ;

PERSICO, MG .

EMBO JOURNAL, 1986, 5 (08) :1849-1855

[35] Glucose-6-phosphate dehydrogenase (G6PD) mutations database: Review of the "old" and update of the new mutations [J].

Minucci, Angelo ;

Moradkhani, Kamran ;

Hwang, Ming Jing ;

Zuppi, Cecilia ;

Giardina, Bruno ;

Capoluongo, Ettore .

BLOOD CELLS MOLECULES AND DISEASES, 2012, 48 (03) :154-165

[36]

NAGARATNAM N, 1969, INDIAN J MED RES, V57, P569

[37] The global prevalence of glucose-6-phosphate dehydrogenase deficiency: A systematic review and meta-analysis [J].

Nkhoma, Ella T. ;

Poole, Charles ;

Vannappagari, Vani ;

Hall, Susan A. ;

Beutler, Ernest .

BLOOD CELLS MOLECULES AND DISEASES, 2009, 42 (03) :267-278

[38] Clinical aspects of hemolysis in patients with P-vivax malaria treated with primaquine, in the Brazilian Amazon [J].

Ramos Junior, Wilson M. ;

Sardinha, Jose F. J. ;

Costa, Monica R. F. ;

Santana, Marli S. ;

Alecrim, Maria G. C. ;

Lacerda, Marcus V. G. .

BRAZILIAN JOURNAL OF INFECTIOUS DISEASES, 2010, 14 (04) :410-412

[39]

Ranawaka RR, 2008, LEPROSY REV, V79, P436

[40] NATURAL-SELECTION OF HEMIZYGOTES AND HETEROZYGOTES FOR G6PD DEFICIENCY IN AFRICA BY RESISTANCE TO SEVERE MALARIA [J].

RUWENDE, C ;

KHOO, SC ;

SNOW, AW ;

YATES, SNR ;

KWIATKOWSKI, D ;

GUPTA, S ;

WARN, P ;

ALLSOPP, CEM ;

GILBERT, SC ;

PESCHU, N ;

NEWBOLD, CI ;

GREENWOOD, BM ;

MARSH, K ;

HILL, AVS .

NATURE, 1995, 376 (6537) :246-249

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