UHPLC-MS/MS method for the quantitation of penicillin G and metabolites in citrus fruit using internal standards

被引:8
作者
Canzani, Daniele [1 ]
Hsieh, Kevin [1 ]
Standland, Matthew [1 ]
Hammack, Walter [1 ]
Aldeek, Fadi [1 ]
机构
[1] Florida Dept Agr & Consumer Serv, Div Food Safety, 3125 Conner Blvd, Tallahassee, FL 32399 USA
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2017年 / 1044卷
关键词
Huanglongbing (HLB); Penicillin G; Metabolites; LC-MS/MS; Internal standards; Method development; BETA-LACTAM ANTIBIOTICS; CANDIDATUS LIBERIBACTER ASIATICUS; TANDEM MASS-SPECTROMETRY; HUMAN PLASMA; LC-MS/MS; GREENING DISEASE; WASTE-WATER; ALLERGY; DEGRADATION; LIBEROBACTER;
D O I
10.1016/j.jchromb.2017.01.012
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Penicillin G has been applied to citrus trees as a potential treatment in the fight against Huanglongbing (HLB). Here, we have developed and validated a method to identify and quantitate penicillin G and two of its metabolites, penillic acid and penilloic acid, in citrus fruit using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). This method improves upon a previous method by incorporating isotopically labeled internal standards, namely, penillic acid-D-5, and penilloic acid-D-5. These standards greatly enhanced the accuracy and precision of our measurements by compensating for recovery losses, degradation, and matrix effects. When 2g of citrus fruit sample is extracted, the limits of detection (LOD) were determined to be 0.1 ng/g for penicillin G and penilloic acid, and 0.25 ng/g for penillic acid. At fortification levels of 0.1, 0.25, 1, and 10 ng/g, absolute recoveries for penillic and penilloic acids were generally between 50-70%. Recoveries corrected with the isotopically labeled standards were approximately 90-110%. This method will be useful for the identification and quantitation of drug residues and their degradation products using isotopically labeled standards and UHPLC-MS/MS. Published by Elsevier B.V.
引用
收藏
页码:87 / 94
页数:8
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