TRPV3 is a calcium-permeable temperature-sensitive cation channel

被引:677
作者
Xu, HX
Ramsey, IS
Kotecha, SA
Moran, MM
Chong, JHA
Lawson, D
Ge, P
Lilly, J
Silos-Santiago, I
Xie, Y
DiStefano, PS
Curtis, R
Clapham, DE
机构
[1] Harvard Univ, Childrens Hosp, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Program Neurosci, Boston, MA 02115 USA
[3] Millennium Pharmaceut Inc, Cambridge, MA 02139 USA
关键词
D O I
10.1038/nature00882
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Transient receptor potential (TRP) proteins are cation-selective channels that function in processes as diverse as sensation and vasoregulation. Mammalian TRP channels that are gated by heat and capsaicin (> 43 degreesC; TRPV1 (ref. 1)), noxious heat (> 52 degreesC; TRPV2 (ref. 2)), and cooling (< 22 degrees C; TRPM8 (refs 3, 4)) have been cloned; however, little is known about the molecular determinants of temperature sensing in the range between similar to 22 degrees C and 40 degrees C. Here we have identified a member of the vanilloid channel family, human TRPV3 (hTRPV3) that is expressed in skin, tongue, dorsal root ganglion, trigeminal ganglion, spinal cord and brain. Increasing temperature from 22 degrees C to 40 degrees C in mammalian cells transfected with hTRPV3 elevated intracellular calcium by activating a nonselective cationic conductance. As in published recordings from sensory neurons, the current was steeply dependent on temperature, sensitized with repeated heating, and displayed a marked hysteresis on heating and cooling(5-10). On the basis of these properties, we propose that hTRPV3 is thermosensitive in the physiological range of temperatures between TRPM8 and TRPV1.
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页码:181 / 186
页数:7
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