Age-related and atherosclerosis-related erythropathy in ApoE/LDLR-/- mice

被引：19

作者：

Dybas, Jakub ^{[1
]}

Bulat, Katarzyna ^{[1
]}

Blat, Aneta ^{[1
,2
]}

Mohaissen, Tasnim ^{[1
,3
]}

Wajda, Aleksandra ^{[1
,4
]}

Mardyla, Mateusz ^{[1
,5
]}

Kaczmarska, Magdalena ^{[1
]}

Franczyk-Zarow, Magdalena ^{[6
]}

Malek, Kamilla ^{[2
]}

Chlopicki, Stefan ^{[1
,7
]}

Marzec, Katarzyna M. ^{[1
]}

机构：

[1] Jagiellonian Univ, Jagiellonian Ctr Expt Therapeut, 14 Bobrzynskiego St, PL-30348 Krakow, Poland

[2] Jagiellonian Univ, Fac Chem, 2 Gronostajowa Str, PL-30387 Krakow, Poland

[3] Jagiellonian Univ, Fac Pharm, Med Coll, PL-30688 Krakow, Poland

[4] AGH Univ Sci & Technol, Fac Mat Sci & Ceram, Mickiewicza 30, PL-30059 Krakow, Poland

[5] Jagiellonian Univ, Univ Sch Phys Educ Krakow, 78 Jana Pawla II St, PL-31571 Krakow, Poland

[6] Univ Agr, Fac Food Technol, Dept Human Nutr & Dietet, 122 Balicka St, PL-30149 Krakow, Poland

[7] Jagiellonian Univ, Dept Expt Pharmacol, Med Coll, 16 Grzegorzecka St, PL-31531 Krakow, Poland

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 2020年 / 1866卷 / 12期

关键词：

Red blood cells (RBCs); RBC membranes; RBC deformability; Fourier transform infrared spectroscopy-attenuated total reflectance; (FTIR-ATR); Raman spectroscopy (RS); Atomic force microscopy (AFM); RED-BLOOD-CELLS; APOLIPOPROTEIN-E-DEFICIENT; DENSITY-LIPOPROTEIN RECEPTOR; ERYTHROCYTE DEFORMABILITY; PLASMA-CHOLESTEROL; MEMBRANE-FLUIDITY; FATTY-ACIDS; EXERCISE; ESTERS; VOLUME;

D O I：

10.1016/j.bbadis.2020.165972

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work we applied a multimodal approach to define the age- and atherosclerosis-related biochemical and functional alterations in red blood cells (RBCs) in ApoE/LDLR-/- mice. Our results revealed that age-related changes in RBCs, such as decreases in RBC deformability and mean height, were more pronounced in ApoE/LDLR-/- mice than in age-matched control mice (C57BL/6J). The decreases in phospholipid content and level of lipid unsaturation were accompanied by an increase in cholesterol esters and esterified lipids in RBC membranes in aged C57BL/6J mice. The age-related decrease in the phospholipid content was more pronounced in ApoE/LDLR-/- mice. In contrast, the increase in the total lipid content in RBC membranes occurred only in ApoE/LDLR-/- mice with advanced atherosclerosis. The age-related alterations also included a decrease in the ratio of turns to a-helices in the secondary structure of hemoglobin (Hb) inside intact RBCs. On the other hand, an increase in the ratio of unordered conformations to alpha-helices of Hb was observed only in ApoE/LDLR-/- mice and occurred already at the age of 5-weeks. This was related to hypercholesterolemia and resulted in an increased oxygen-carrying capacity. In conclusion, progressive mechanical and functional alterations of RBCs in aged ApoE/LDLR-/- mice were more pronounced than in age-matched C57BL/6J mice. Although, several biochemical changes in RBCs in aged ApoE/LDLR-/- mice recapitulated age-dependent changes observed in control mice, some biochemical features of RBC membranes attributed to hypercholesterolemia were distinct and could contribute to the accelerated deterioration of RBC function in ApoE/LDLR-/- mice.

引用

页数：11

共 63 条

[1] AGE-RELATED-CHANGES OF ERYTHROCYTE-MEMBRANE IN THE SENESCENCE-ACCELERATED MOUSE [J].