Obtaining lipases from byproducts of orange juice processing

被引:22
作者
Okino-Delgado, Clarissa Hamaio [1 ]
Fleuri, Luciana Francisco [1 ]
机构
[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Chem & Biochem, BR-18618000 Botucatu, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
Lipase; Byproducts; Orange; Core; Frit; Peel; DIETARY FIBER; PINEAPPLE; FRUIT;
D O I
10.1016/j.foodchem.2014.04.090
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The presence of lipases was observed in three byproducts of orange juice processing: peel, core and frit. The enzymes were characterised biochemically over a wide pH range from neutral (6-7) to alkaline (8-9). The optimal temperature for the activity of these byproducts showed wide range at 20 degrees C to 70 degrees C, indicating fairly high thermostability. The activities were monitored on p-NP-butyrate, p-NP-laurate and p-NP-palmitate. For the first time, lipase activity was detected in these residues, reaching 68.5 lipase U/g for the crude extract from fractions called frit. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:103 / 107
页数:5
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