The immunological activity of Lycium barbarum polysaccharides liposome in vitro and adjuvanticity against PCV2 in vivo

被引：63

作者：

Bo, Ruonan ^{[1
]}

Zheng, Sisi ^{[1
]}

Xing, Jie ^{[1
]}

Luo, Li ^{[1
]}

Niu, Yale ^{[1
]}

Huang, Yee ^{[1
]}

Liu, Zhenguang ^{[1
]}

Hu, Yuanliang ^{[1
]}

Liu, Jiaguo ^{[1
]}

Wu, Yi ^{[1
]}

Wang, Deyun ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Inst Tradit Chinese Vet Med, Nanjing 210095, Jiangsu, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 85卷

基金：

中国国家自然科学基金;

关键词：

Lycium barbarum polysaccharides liposome; Immunological activity; Vaccine adjuvant; Immune responses; TOLL-LIKE RECEPTORS; REGULATORY T-CELLS; VACCINE ADJUVANTS; ASTRAGALUS POLYSACCHARIDE; IMMUNE-RESPONSE; DELIVERY; MACROPHAGES; NANOPARTICLES; IMPROVE;

D O I：

10.1016/j.ijbiomac.2015.12.089

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In previous researches, the results showed that Lycium barbarum polysaccharides (LBP) encapsulated with liposome could enhance the immune activity of LBP. Therefore, the present study was designed to investigate the effects of LBPL on spleen lymphocytes and macrophages of mice in vitro and evaluate the immunological adjuvant activity of PCV2 vaccine in vivo. The results showed that LBPL could significantly promote splenocyte proliferation synergistically with PHA or LPS, increase the ratio of CD4(+) to CD8(+) T cells and promote the cytokine secretion of macrophages; enhance PCV2-specific IgG antibody responses, promote Th1 cytokines (IFN-gamma and TNF-alpha) and Th2 cytokine (IL-4) secretion. The histomorphological observation of spleen demonstrated that LBPL as a vaccine adjuvant also has good improvement and stimulating effect on the immune organ. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：294 / 301

页数：8

共 43 条

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