Oxidative changes in the blood and serum albumin differentiate rats with monoarthritis and polyarthritis

被引：34
作者：
Bracht, Adelar [1 ]
Silveira, Sandra Silva [1 ]
Castro-Ghizoni, Cristiane Vizioli [1 ]
Sa-Nakanishi, Anacharis Babeto [1 ]
Neves Oliveira, Marcia Rosangela [2 ]
Bersani-Amado, Ciomar Aparecida [3 ]
Peralta, Rosane Marina [1 ]
Comar, Jurandir Fernando [1 ]
机构：
[1] Univ Estadual Maringa, Dept Biochem, BR-87020900 Maringa, Parana, Brazil
[2] Univ Estadual Maringa, Dept Clin Anal, BR-87020900 Maringa, Parana, Brazil
[3] Univ Estadual Maringa, Dept Pharmacol & Therapeut, BR-87020900 Maringa, Parana, Brazil
来源：
SPRINGERPLUS | 2016年 / 5卷
关键词：
Chronic inflammation; Adjuvant-induced arthritis; Antioxidant status; Serum albumin oxidation; ADJUVANT-INDUCED ARTHRITIS; TOTAL ANTIOXIDANT CAPACITY; RHEUMATOID-ARTHRITIS; LIPID-PEROXIDATION; CARDIOVASCULAR-DISEASE; HUMAN PLASMA; PROTEIN; STRESS; REDOX; STATE;
D O I：
10.1186/s40064-016-1671-1
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Adjuvant arthritis in rats, as rheumatoid arthritis in humans, may be of greater or lesser severity, namely polyarthritis and monoarthritis, respectively. The present study was planned to evaluate the oxidative changes in the blood and specifically in the serum albumin of rats with adjuvant-induced mono-and poly-arthritis. Total antioxidant capacity, thiols, carbonyl groups, albumin, uric acid and ascorbic acid were measured in the total serum. The specific oxidative status of albumin was also measured after separation by affinity chromatography. All serum oxidative parameters were close to normal in monoarthritic rats with the exception of the ascorbic acid concentration, which was 23 % lower, and albumin carbonyl groups, which were 64 % higher. Many modifications were found in polyarthritic rats, specially the ascorbic acid concentration (35 % lower) and albumin carbonyl groups (102 % higher). The results revealed that the levels of ascorbic acid in the serum and carbonyl groups in the albumin molecule can be regarded as indicators of the severity of arthritis since they were modified by both monoarthritis and polyarthritis, but to different degrees.
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页码：1 / 14
页数：14
相关论文
共 53 条
[1]   Carvedilol alleviates adjuvant-induced arthritis and subcutaneous air pouch edema: Modulation of oxidative stress and inflammatory mediators [J].
Arab, Hany H. ;
El-Sawalhi, Maha M. .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2013, 268 (02) :241-248
[2]   ALTERED THIOL STATUS IN PATIENTS WITH RHEUMATOID-ARTHRITIS [J].
BANFORD, JC ;
BROWN, DH ;
HAZELTON, RA ;
MCNEIL, CJ ;
SMITH, WE ;
STURROCK, RD .
RHEUMATOLOGY INTERNATIONAL, 1982, 2 (03) :107-111
[3]   Animal models of arthritis: Relevance to human disease [J].
Bendele, A ;
McComb, J ;
Gould, T ;
McAbee, T ;
Sennello, G ;
Chlipala, E ;
Guy, M .
TOXICOLOGIC PATHOLOGY, 1999, 27 (01) :134-142
[4]   Effects of simvastatin, atorvastatin, ezetimibe, and ezetimibe plus simvastatin combination on the inflammatory process and on the liver metabolic changes of arthritic rats [J].
Bracht, Livia ;
Barbosa, Carmem Patricia ;
Caparroz-Assef, Silvana Martins ;
Nakamura Cuman, Roberto Kenji ;
Ishii-Iwamoto, Emy Luiza ;
Bracht, Adelar ;
Bersani-Amado, Ciomar Aparecida .
FUNDAMENTAL & CLINICAL PHARMACOLOGY, 2012, 26 (06) :722-734
[5]   Oxidative state of the liver of rats with adjuvant-induced arthritis [J].
Comar, Jurandir Fernando ;
de Sa-Nakanishi, Anacharis Babeto ;
de Oliveira, Andrea Luiza ;
Nogueira Wendt, Mariana Marques ;
Bersani Amado, Ciomar Aparecida ;
Ishii Iwamoto, Emy Luiza ;
Peralta, Rosane Marina ;
Bracht, Adelar .
FREE RADICAL BIOLOGY AND MEDICINE, 2013, 58 :144-153
[6]  
Cylmik B, 2010, ADV MED SCI, V55, P80
[7]   Correlation of oxidant status with oxidative tissue damage in patients with rheumatoid arthritis [J].
Datta, Suhana ;
Kundu, Sunanda ;
Ghosh, Parasar ;
De, Soumita ;
Ghosh, Alakendu ;
Chatterjee, Mitali .
CLINICAL RHEUMATOLOGY, 2014, 33 (11) :1557-1564
[8]   A DISCRETE ADJUVANT-INDUCED MONOARTHRITIS IN THE RAT - EFFECTS OF ADJUVANT DOSE [J].
DONALDSON, LF ;
SECKL, JR ;
MCQUEEN, DS .
JOURNAL OF NEUROSCIENCE METHODS, 1993, 49 (1-2) :5-10
[9]   A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation [J].
Erel, O .
CLINICAL BIOCHEMISTRY, 2004, 37 (04) :277-285
[10]   Human serum albumin: From bench to bedside [J].
Fanali, Gabriella ;
di Masi, Alessandra ;
Trezza, Viviana ;
Marino, Maria ;
Fasano, Mauro ;
Ascenzi, Paolo .
MOLECULAR ASPECTS OF MEDICINE, 2012, 33 (03) :209-290
123456