Metabolomics and Isotope Tracing

被引：603

作者：

Jang, Cholsoon ^{[1
,2
]}

Chen, Li ^{[1
,2
]}

Rabinowitz, Joshua D. ^{[1
,2
]}

机构：

[1] Princeton Univ, Lewis Sigler Inst Integrat Genom, Washington Rd, Princeton, NJ 08544 USA

[2] Princeton Univ, Dept Chem, Washington Rd, Princeton, NJ 08544 USA

来源：

CELL | 2018年 / 173卷 / 04期

基金：

美国国家卫生研究院;

关键词：

CELL LUNG-CANCER; MASS-SPECTROMETRY; NMR-SPECTROSCOPY; METABOLITE CONCENTRATIONS; LIQUID-CHROMATOGRAPHY; GLUTAMINE-METABOLISM; ESCHERICHIA-COLI; TUMOR-CELLS; PROTEIN; FLUX;

D O I：

10.1016/j.cell.2018.03.055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Great strides have been made over the past decade toward comprehensive study of metabolism. Mass spectrometry (MS) has played a central role by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. Here, we describe the basics of metabolite measurement by MS, including sample preparation, metabolomic analysis, and data interpretation. In addition, drawing on examples of successful experiments, we highlight the ways in which metabolomics and isotope tracing can illuminate biology.

引用

页码：822 / 837

页数：16

共 99 条

[61] U13C cell extract of Pichia pastoris - a powerful tool for evaluation of sample preparation in metabolomics [J].

Neubauer, Stefan ;

Haberhauer-Troyer, Christina ;

Klavins, Kristaps ;

Russmayer, Hannes ;

Steiger, Matthias G. ;

Gasser, Brigitte ;

Sauer, Michael ;

Mattanovich, Diethard ;

Hann, Stephan ;

Koellensperger, Gunda .

JOURNAL OF SEPARATION SCIENCE, 2012, 35 (22) :3091-3105

[62] A Branched-Chain Amino Acid-Related Metabolic Signature that Differentiates Obese and Lean Humans and Contributes to Insulin Resistance [J].

Newgard, Christopher B. ;

An, Jie ;

Bain, James R. ;

Muehlbauer, Michael J. ;

Stevens, Robert D. ;

Lien, Lillian F. ;

Haqq, Andrea M. ;

Shah, Svati H. ;

Arlotto, Michelle ;

Slentz, Cris A. ;

Rochon, James ;

Gallup, Dianne ;

Ilkayeva, Olga ;

Wenner, Brett R. ;

Yancy, William S., Jr. ;

Eisenson, Howard ;

Musante, Gerald ;

Surwit, Richard S. ;

Millington, David S. ;

Butler, Mark D. ;

Svetkey, Laura P. .

CELL METABOLISM, 2009, 9 (04) :311-326

[63] Using Genome-scale Models to Predict Biological Capabilities [J].

O'Brien, Edward J. ;

Monk, Jonathan M. ;

Palsson, Bernhard O. .

CELL, 2015, 161 (05) :971-987

[64] Impact of Anesthesia and Euthanasia on Metabolomics of Mammalian Tissues: Studies in a C57BL/6J Mouse Model [J].

Overmyer, Katherine A. ;

Thonusin, Chanisa ;

Qi, Nathan R. ;

Burant, Charles F. ;

Evans, Charles R. .

PLOS ONE, 2015, 10 (02)

[65]

Park JO, 2016, NAT CHEM BIOL, V12, P482, DOI [10.1038/NCHEMBIO.2077, 10.1038/nchembio.2077]

[66] An integrated genomic analysis of human glioblastoma Multiforme [J].

Parsons, D. Williams ;

Jones, Sian ;

Zhang, Xiaosong ;

Lin, Jimmy Cheng-Ho ;

Leary, Rebecca J. ;

Angenendt, Philipp ;

Mankoo, Parminder ;

Carter, Hannah ;

Siu, I-Mei ;

Gallia, Gary L. ;

Olivi, Alessandro ;

McLendon, Roger ;

Rasheed, B. Ahmed ;

Keir, Stephen ;

Nikolskaya, Tatiana ;

Nikolsky, Yuri ;

Busam, Dana A. ;

Tekleab, Hanna ;

Diaz, Luis A., Jr. ;

Hartigan, James ;

Smith, Doug R. ;

Strausberg, Robert L. ;

Marie, Suely Kazue Nagahashi ;

Shinjo, Sueli Mieko Oba ;

Yan, Hai ;

Riggins, Gregory J. ;

Bigner, Darell D. ;

Karchin, Rachel ;

Papadopoulos, Nick ;

Parmigiani, Giovanni ;

Vogelstein, Bert ;

Velculescu, Victor E. ;

Kinzler, Kenneth W. .

SCIENCE, 2008, 321 (5897) :1807-1812

[67] Acidic acetonitrile for cellular metabolome extraction from Escherichia coli [J].

Rabinowitz, Joshua D. ;

Kimball, Elizabeth .

ANALYTICAL CHEMISTRY, 2007, 79 (16) :6167-6173

[68] Genetically encoded sensors enable real-time observation of metabolite production [J].

Rogers, Jameson K. ;

Church, George M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (09) :2388-2393

[69] Assignment of endogenous substrates to enzymes by global metabolite profiling [J].

Saghatelian, A ;

Trauger, SA ;

Want, EJ ;

Hawkins, EG ;

Siuzdak, G ;

Cravatt, BF .

BIOCHEMISTRY, 2004, 43 (45) :14332-14339

[70] In Vivo Imaging of Glutamine Metabolism to the Oncometabolite 2-Hydroxyglutarate in IDH1/2 Mutant Tumors [J].

Salamanca-Cardona, Lucia ;

Shah, Hardik ;

Poot, Alex J. ;

Correa, Fabian M. ;

Di Gialleonardo, Valentina ;

Lui, Hui ;

Miloushev, Vesselin Z. ;

Granlund, Kristin L. ;

Tee, Sui S. ;

Cross, Justin R. ;

Thompson, Craig B. ;

Keshari, Kayvan R. .

CELL METABOLISM, 2017, 26 (06) :830-+

← 1 2 3 4 5 6 7 8 9 10 →