Metabolomics and Isotope Tracing

被引：603
作者：
Jang, Cholsoon [1 ,2 ]
Chen, Li [1 ,2 ]
Rabinowitz, Joshua D. [1 ,2 ]
机构：
[1] Princeton Univ, Lewis Sigler Inst Integrat Genom, Washington Rd, Princeton, NJ 08544 USA
[2] Princeton Univ, Dept Chem, Washington Rd, Princeton, NJ 08544 USA
来源：
CELL | 2018年 / 173卷 / 04期
基金：
美国国家卫生研究院;
关键词：
CELL LUNG-CANCER; MASS-SPECTROMETRY; NMR-SPECTROSCOPY; METABOLITE CONCENTRATIONS; LIQUID-CHROMATOGRAPHY; GLUTAMINE-METABOLISM; ESCHERICHIA-COLI; TUMOR-CELLS; PROTEIN; FLUX;
D O I：
10.1016/j.cell.2018.03.055
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Great strides have been made over the past decade toward comprehensive study of metabolism. Mass spectrometry (MS) has played a central role by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. Here, we describe the basics of metabolite measurement by MS, including sample preparation, metabolomic analysis, and data interpretation. In addition, drawing on examples of successful experiments, we highlight the ways in which metabolomics and isotope tracing can illuminate biology.
引用
收藏
页码：822 / 837
页数：16
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