Proteomic identification of processes and pathways characteristic of osmoregulatory tissues in spiny dogfish shark (Squalus acanthias)

被引:25
作者
Lee, Jinoo
Valkova, Nelly
White, Mark P.
Kultz, Dietmar
机构
[1] Univ Calif Davis, Dept Anim Sci, Physiol Genom Grp, Davis, CA 95616 USA
[2] Mt Desert Isl Biol Lab, Salsbury Cove, ME USA
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS | 2006年 / 1卷 / 03期
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
shark; osmoregulation; proteomics; pathway analysis; gene ontology; tissue-specificity;
D O I
10.1016/j.cbd.2006.07.001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We used dogfish shark (Squalus. acanthias) as a model for proteome analysis of six different tissues to evaluate tissue-specific protein expression on a global scale and to deduce specific functions and the relatedness of multiple tissues from their proteomes. Proteomes of heart, brain, kidney, intestine, gill, and rectal gland were separated by two-dimensional gel electrophoresis (2DGE), gel images were matched using Delta 2D software and then evaluated for tissue-specific proteins. Sixty-one proteins (4%) were found to be in only a single type of tissue and 535 proteins (36%) were equally abundant in all six tissues. Relatedness between tissues was assessed based on tissue-specific expression patterns of all 1465 consistently resolved protein spots. This analysis revealed that tissues with osmoregulatory function (kidney, intestine, gill, rectal gland) were more similar in their overall proteomes than non-osmoregulatory tissues (heart, brain). Sixty-one proteins were identified by MALDI-TOF/TOF mass spectrometry and biological functions characteristic of osmoregulatory tissues were derived from gene ontology and molecular pathway analysis. Our data demonstrate that the molecular machinery for energy and urea metabolism and the Rho-GTPase/cytoskeleton pathway are enriched in osmoregulatory tissues of sharks. Our work provides a strong rationale for further study of the contribution of these mechanisms to the osmoregulation of marine sharks. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:328 / 343
页数:16
相关论文
共 42 条
[1]   Angiotensin and angiotensin receptors in cartilaginous fishes [J].
Anderson, WG ;
Cerra, MC ;
Wells, A ;
Tierney, ML ;
Tota, B ;
Takei, Y ;
Hazon, N .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2001, 128 (01) :31-40
[2]   Human tissue profiling with multidimensional protein identification technology [J].
Cagney, G ;
Park, S ;
Chung, C ;
Tong, B ;
O'Dushlaine, C ;
Shields, DC ;
Emili, A .
JOURNAL OF PROTEOME RESEARCH, 2005, 4 (05) :1757-1767
[3]   Osmoregulation of endothelial nitric-oxide synthase gene expression in inner medullary collecting duct cells - Role in activation of the type a natriuretic peptide receptor [J].
Chen, SC ;
Cao, L ;
Intengan, HD ;
Humphreys, M ;
Gardner, DG .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (36) :32498-32504
[4]   Transcription, genomes, function [J].
Cho, RJ ;
Campbell, MJ .
TRENDS IN GENETICS, 2000, 16 (09) :409-415
[5]   Phosphatidylserine decarboxylases as genetic and biochemical tools for studying phospholipid traffic [J].
Choi, JY ;
Wu, WI ;
Voelker, DR .
ANALYTICAL BIOCHEMISTRY, 2005, 347 (02) :165-175
[6]   Role of nitric oxide in larval and juvenile fish [J].
Eddy, FB .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2005, 142 (02) :221-230
[7]   Rho's role in cell volume: sensing, strutting, or signaling? Focus on "Hyperosmotic stress activates Rho: differential involvement in Rho kinase-dependent MLC phosphorylation and NKCC activation" [J].
Eggermont, J .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2003, 285 (03) :C509-C511
[8]  
EPSTEIN FH, 1983, J EXP BIOL, V106, P25
[9]   Urea based osmoregulation and endocrine control in elasmobranch fish with special reference to euryhalinity [J].
Hazon, N ;
Wells, A ;
Pillans, RD ;
Good, JP ;
Anderson, WG ;
Franklin, CE .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2003, 136 (04) :685-700
[10]  
Karnaky KJ, 2002, FASEB J, V16, pA1154